摘要
近年来,海水青鳉(Oryzias melastigma)已经被公认为海洋生态毒理学研究中的一种模式生物,其具有与淡水模式生物斑马鱼(Danio rerio)类似的研究特征优势,如世代时间短(3~4个月)、每日产卵、成鱼尺寸小(2.5~3.5 cm)、胚胎透明、性别二态性以及易于进行实验室规模化养殖等。在过去十余年中,国内外的研究者们对海水青鳉进行了广泛的基因组和蛋白质组研究。这些组学数据能够进一步帮助我们在分子层面了解环境胁迫对海洋生物的潜在影响及其可能的毒性机制。在这篇综述中,我们归纳了海水青鳉作为海洋生态毒理学研究模型的优势,介绍了当前用于海水青鳉基因/蛋白质组学的技术方法,整理了基因/蛋白质组学在研究不同种类的海洋环境压力源对海水青鳉毒性效应的应用现状。最后,我们对未来海水青鳉的研究提出了一定的预期与展望。
In recent years,marine medaka(Oryzias melastigma)has been recognized as a model organism for marine ecotoxicology research.It has similar research superiorities as the freshwater model organism zebrafish(Danio rerio),including short generation time(3~4 months),daily oviposition,small adult size(2.5~3.5 cm),transparent embryo,sexual dimorphism,and easy lab-scale cultivation.In the past few decades,domestic and foreign researchers have conducted extensive genomic and proteomic studies of O.melastigma.These omics data can further help us understand the potential impacts of environmental stress on marine organisms at the molecular level and their possible toxic mechanisms.In this review,we summarized the advantages of O.melastigma as a marine ecotoxicological research model,introduced the current technical methods for Oryzias melastigma genomics and proteomics studies,and sorted out the application status of genomics and proteomics in studying the toxic effects of different types of the marine environmental pressure source on Oryzias melastigma.Finally,we put forward some expectations and prospects for the future research of Oryzias melastigma.
作者
孙哲诚
梁川
李乾永
马正茁
徐炎华
刘志英
Sun Zhecheng;Liang Chuan;Li Qianyong;Ma Zhengzhuo;Xu Yanhua;Liu Zhiying(School of Environmental Science and Engineering,Nanjing Tech University,Nanjing 211816,China)
出处
《生态毒理学报》
CAS
CSCD
北大核心
2023年第2期75-96,共22页
Asian Journal of Ecotoxicology
基金
国家“十三五”重点研发课题(2017YFB0602500)。
