摘要
[目的]为了分析河西走廊地区犊牛腹泻致病性大肠杆菌携带毒力基因和耐药性情况,[方法]2020-2021年在河西走廊地区采集患腹泻病犊牛的粪便、肛拭子及肝脏等病料组织279份,采用人工感染试验动物、PCR方法和K-B药敏纸片法分别检测犊牛腹泻性大肠杆菌致病性、毒力因子和耐药性。[结果]结果表明,分离得到了126株大肠杆菌,其中79株犊牛腹泻性大肠杆菌能引起小鼠死亡;分离的79株致病性大肠杆菌的毒力基因crl、irp2、fimH、papC、K88、K99、stx1、stx2检测率在40.5%~100%之间,其他毒力基因检测率在15.2%~34.2%之间;分离的79株致病性大肠杆菌对氨苄西林、阿莫西林、新霉素等8种药物的耐药率在49.4%~96.2%之间,对其他药物的耐药率在5.1%~32.9%之间。[结论]从河西走廊地区患腹泻病犊牛病料组织中分离得到79株致病性大肠杆菌,这些菌株携带多种毒力基因,对临床中常用的抗菌药物产生了耐药性。
[Objective]In order to analyze the virulence gene and drug resistance of calf diarrhea pathogenic E.coli in Hexi Corridor region.[Method]From 2020 to 2021,279 diseased tissues such as feces,anal swabs and liver of calves suffering from diarrhea disease were collected in Hexi Corridor region.The pathogenicity,virulence factors and drug resistance of calf diarrhea E.coli were detected by artificial infection test animals,PCR method and K-B drug sensitive paper method.[Result]The results showed that 126 strains of E.coli were isolated,among which 79 strains of calf diarrhea E.coli could cause death of mice.The detection rates of virulence genes crl,irp2,fimH,papC,K88,K99,stx1 and stx2 of 79 strains of pathogenic E.coli were 40.5%~100%,and the detection rates of other virulence genes were 15.2%~34.2%.The resistance rate of 79 strains of pathogenic E.coli isolated to 8 drugs,such as ampicillin,amoxicillin and neomycin,was 49.4%~96.2%,and the resistance rate to other drugs was 5.1%~32.9%.[Conclusion]79 strains of pathogenic E.coli were isolated from the diseased tissues of calves suffering from diarrhea in the Hexi Corridor.79 strains of pathogenic%E.coli carry multiple virulence genes and are resistant to commonly used antibiotics in clinical practice.
作者
马忠
MA Zhong(Gansu Animal Disease Prevention and Control Center,Lanzhou 730046)
出处
《中国牛业科学》
2023年第2期29-33,共5页
China Cattle Science
关键词
犊牛
腹泻
大肠杆菌
致病性
毒力基因
耐药性
calf
diarrhea
E.coli
pathogenicity
virulence gene
drug resistance
