摘要
目的:探讨Notch1及Hes1在弥漫大B细胞淋巴瘤(DLBCL)组织中的表达及其与患者临床特征的相关性。方法:采用免疫组织化学法对比观察54例DLBCL及20例淋巴结炎组织中Notch1、Hes1的表达及与患者临床特征的关系。同时利用Oncomine数据库数据,分析DLBCL组织中Notch1、Hes1 mRNA和DNA水平。结果:免疫组化结果显示,Notch1、Hes1在DLBCL患者中的阳性表达率高于对照组(P<0.05)。Notch1表达与DLBCL患者临床B症状、Ann Arbor分期、淋巴细胞计数及乳酸脱氢酶水平密切相关(P<0.05),而Hes1在B症状患者中显著高表达(P<0.05)。在DLBCL组织中,Notch^(+)/Hes1^(+)表达21例(38.9%),Notch1与Hes1表达具有相关性(r=0.296,P<0.05)。生信分析Oncomine数据库中多个数据集发现,在Brune数据集中Notch1、Hes1 mRNA表达显著高于对照组织(P<0.05)。结论:DLBCL组织中Notch1、Hes1表达明显高于淋巴结炎组织,其表达与B症状、Ann Arbor分期等具有相关性,提示Notch1、Hes1在DCBCL的发生发展中发挥重要作用。
Objective: To investigate the expressions of Notch1 and Hes1 in diffuse large B-cell lymphoma(DLBCL), and their correlations with clinical features. Methods: Immunohistochemistry(IHC) was performed on DLBCL samples(54 cases) and lymphadenitis tissues(20 cases) to evaluate the expressions of Notch1 and Hes1, and analyze their correlations with clinical characteristics of patients. Based on Oncomine database, the expressions of Notch1 and Hes1 mRNA and DNA were also explored. Results: IHC result showed that the positive expression rates of Notch1 and Hes1 in DLBCL patients were significantly higher than those in the control group(P<0.05). In DLBCL patients, the expression of Notch1 was closely associated with B symptoms, Ann Arbor stage, lymphocyte count and the level of lactate dehydrogenase(P<0.05), while the expression level of Hes1 was significantly higher in patients with B symptoms(P<0.05). Notch^(+)/Hes1^(+)expression was found in 21 DLBCL tissues(38.9%), and there was a correlation between Notch1 and Hes1 expression(r=0.296, P<0.05). Bioinformatics analysis(Oncomine database) showed that the mRNA expressions of Notch1 and Hes1 in the Brune dataset were significantly higher than those in the control tissues(P<0.05). Conclusion: The expressions of Notch1 and Hes1 in DLBCL are significantly higher than those in lymphadenitis, and correlated with B symptoms and Ann Arbor stage, suggesting that Notch1 and Hes1 play important roles in the occurrence and development of DLBCL.
作者
刘艳
徐沙沙
王晓宁
郑娜
徐梦瑶
李杰
LIU Yan;XU Sha-Sha;WANG Xiao-Ning;ZHENG Na;XU Meng-Yao;LI Jie(Department of Postgraduate of Hebei North University,Zhangjiakou O75000,Hebei Province,China;Department of Hematology Hebei General Hospital,Shijiazhuang 050000,Hebei Province,China;Department 4,The Second Affiliated Hospital of Luohe Medical College,Luohe 462300,Henan Province,China)
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2023年第3期714-721,共8页
Journal of Experimental Hematology
基金
2020年河北省政府资助临床医学优秀人才培养项目(202013)。
