摘要
目的分析丙泊酚调控脊髓环磷酸腺苷/蛋白激酶A-磷酸化环磷酸腺苷反应元件结合蛋白-脑源性神经营养因子(cAMP/PKA-CREB-BDNF)通路对大鼠神经元凋亡、坐骨神经阻滞效果的影响。方法选取40只SPF级Wistar雄性大鼠,其中对照组、假手术组、低剂量组、高剂量组各10只,空白组不做任何处理,假手术组进行手术处理和0.9%氯化钠溶液注射,低剂量组在假手术的基础上注射丙泊酚10 mg/kg;高剂量组注射丙泊酚30 mg/kg。结果与空白组相比,假手术组、低剂量组、高剂量组SOD活性降低(P<0.05);MDA含量、海马神经元凋亡率、各时间点MPE、MPE均升高以及cAMP、PKA、CREB、BDNF表达均降低(P<0.05)。与假手术组相比,低剂量组、高剂量组MDA含量降低(P<0.05);SOD活性、海马神经元凋亡率、各时间点MPE以及10、20、30、60、90、120 min时间点MPE均升高、150、180 min时间点MPE均降低(P<0.05);cAMP、PKA、CREB、BDNF表达均降低(P<0.05)。与低剂量组相比,高剂量组MDA含量降低(P<0.05);SOD活性、海马神经元凋亡率、各时间点MPE以及150、180min时间点EPT均升高;10、20、30、60、90、120 min时间点EPT均降低,(P<0.05);cAMP、PKA、CREB、BDNF表达均降低(P<0.05)。结论丙泊酚能够增强坐骨神经阻滞效果,降低神经元凋亡率,起保护作用可能与丙泊酚调控cAMP/PKA-CREB-BDNF通路有关。
Objective To analyze the effect of propofol on neuronal apoptosis and sciatic nerve block in rats by regulating the cyclic adenosine monophosphate(cAMP)/protein kinase A(PKA)/cAMP response element binding protein(CREB)/brain-derived neurotrophic factor(BDNF)signaling pathway.Methods A total of 40 male Wistar rats at the SPF level were randomly allocated to control group,sham operation group,low-dose group and high-dose group,with 10 in each group.Rats in control group were treated with blank control,and those in the latter three groups were surgically treated and administrated with 0.9%sodium chloride solution,10mg/kg propofol and 30mg/kg propofol,respectively.Results Compared with those of blank group,significantly decreased superoxide dismutase(SOD)activity,increased malondialdehyde(MDA)content,apoptotic rate of hippocampal neurons and elevated plus maze test(EPT)and maximal potential analgesic efficacy(MPE)at each time point,and downregulated cAMP,PKA,CREB and BDNF were detected in sham operation group,low-dose group and high-dose group(all P<0.05).Compared with those of sham operation group,significantly decreased MDA content and EPT at 150 and 180min,as well as increased SOD activity,apoptotic rate of hippocampal neurons,MPE at each time point,and EPT at 10,20,30,60,90,and 120min,and downregulated cAMP,PKA,CREB and BDNF were detected in low-dose group and high-dose group(all P<0.05).Compared with those of low-dose group,significantly decreased MDA content and EPT at 10,20,30,60,90,and 120min,increased SOD activity,apoptotic rate of hippocampal neurons,MPE at each time point,and EPT at 150 and 180 min,and downregulated cAMP,PKA,CREB and BDNF were detected in high-dose group(all P<0.05).Conclusion Propofol can enhance the sciatic nerve block effect and reduce the rate of neuronal apoptosis by regulating the cAMP/PKA-CREB-BDNF signaling pathway.
作者
吴帮林
朱荣誉
吴述轩
朱贤林
WU Banglin;ZHU Rongyu;WU Shuxuan(Department of Anesthesiology,the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture,Hubei,Enshi 445000,China)
出处
《河北医药》
CAS
2023年第10期1445-1449,共5页
Hebei Medical Journal
基金
湖北省自然科学基金面上项目(编号:2016CFB368)。
