摘要
苯丙氨酸解氨酶是催化L-苯丙氨酸脱氨以进入苯丙烷途径第一步反应的酶,其对植物生长调节和抗逆反应都有着重要作用。从全基因组层次分析青蒿苯丙氨酸解氨酶基因家族的进化关系,可为相关基因功能研究提供线索。本研究利用HMMER和blastp从青蒿基因组中筛选苯丙氨酸解氨酶基因家族成员,并对其序列特征、进化关系以及表达模式等进行分析。最终从青蒿基因组中筛选到了6个苯丙氨酸解氨酶基因家族成员,其编码蛋白的氨基酸残基数量范围为446~716,蛋白分子量范围为49 559.81~77 621.57 kD,等电点(pI)范围为5.41~5.92,蛋白结构预测显示这些蛋白皆为亲水性蛋白;进化分析结果表明,6个AaPAL蛋白均与双子叶植物PAL蛋白的亲缘关系较近,同时这些蛋白序列具有保守的基序;通过对其顺式作用元件分析得知,苯丙氨酸解氨酶基因成员的启动子区域内含有较多的光响应、逆境胁迫响应、激素响应和生长发育相关的元件;青蒿的6个苯丙氨酸解氨酶基因在不同光处理下有着不同的表达方式;通过分析6个苯丙氨酸解氨酶基因的序列特征和表达模式,为深入研究该基因家族成员的功能及其对光调控下黄酮化合物生物合成调控机制提供参考。
Phenylalanine ammonia lyase is an enzyme that catalyzes the deamination of L-phenylalanine to enter the first step reaction in the phenylpropanoid pathway,which is important for both plant growth regulation and stress resistance.Analysis of the evolutionary relationships of the phenylalanine ammonia lyase gene family of Artemisia annua from a genome-wide hierarchy may provide clues for functional studies of related genes.In this study,we used HMMER and blastp to screen the members of the phenylalanine ammonia lyase gene family from the Artemisia annua genome and analyzed their sequence characteristics,evolutionary relationships,as well as expression patterns.Finally,six phenylalanine ammonia lyase gene family members were screened from the Artemisia annua genome,and the number of amino acid residues of the encoded proteins ranged from 446 to 716,the protein molecular weight ranged from 49559.81 to 77621.57 kD,the isoelectric point(pl)ranged from 5.41 to 5.92,and protein structure prediction revealed that these proteins were hydrophilic;Evolutionary analysis results showed that all six AaPAL proteins were closely related to the relatives of the dicotyledonous pal proteins,and at the same time,these protein sequences had conserved motifs;From the analysis of its cis acting elements,it is known that the promoter regions of some phenylalanine ammonia lyase gene members contained many elements related to light response,stress response to adversity,hormone response and growth and development;The six phenylalanine ammonia lyase genes of A.annua had different expression patterns under different light treatments.by analyzing the sequences and expression patterns of six phenylalanine ammonia lyase genes,we provide further insights into the functions of members of this gene family and their regulatory mechanisms on flavonoid biosynthesis under light regulation.
作者
戢强强
陈伟达
陈敬东
王宇航
余忆
曾长立
Ji Qiangqiang;Chen Weida;Chen Jingdong;Wang Yuhang;Yu Yi;Zeng Changli(Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin,School of Life Science,Jianghan University,Wuhan,430056)
出处
《分子植物育种》
CAS
北大核心
2023年第11期3544-3551,共8页
Molecular Plant Breeding
基金
国家重点研发计划项目(2016YFD0100202-25)
湖北省汉江流域特色生物资源保护开发与利用工程技术研究中心开放基金项目(06450003)共同资助。
