摘要
本研究采用高通量Illumina转录组测序技术对‘万海4号’(WH4)、‘博白大果’(BBDG)、‘长林40’(CL40)、‘岑软’(CR)和‘红花油茶’(HH)五个不同种质的油茶进行转录组测序并进行差异比较分析。结果表明,WH4、BBDG、CL40、CR以及HH通过组装分别获得了71 852、111 693、75 436、15 899和96 205个Unigenes,将所有Unigenes与公共数据库(Nr, Swiss-Prot, KOG, KEGG)进行相似性比对并注释,五种油茶注释基因所占百分比依次为65.51%、46.41%、60.57%、91.94%、54.98%;KEGG富集分析表明WH4、BBDG、CL40和HH中基因丰度最高的都是核糖体途径,而CR中基因丰度最高的是碳代谢途径;GO富集分析显示,WH4、BBDG、CL40、CR和HH基因分布结果一致。结合KEGG、GO和KOG富集分析,绘制出了油茶三萜皂苷生物合成途径通路图,筛选出该途径中的关键酶及相关基因,并对其进行了差异分析。该研究结果完善了油茶三萜皂苷生物合成途径相关基因的表达特征,可为油茶三萜皂苷的生物合成提供科学依据。
In this study,high-throughput Illumina transcriptome sequencing technology was used to perform trans-criptome sequencing and comparative analysis from Camellia oleifera of different species,such as Camellia oleifera Abel.(CL40 and CR),Camellia vietnamensis Huang(WH4),Camellia gigantocarpa(BBDG),and Camellia che-kiangoleosa(HH).The results showed that 71852,111693,75436,15899 and 96205 Unigenes were respectively obtained in WH4,BBDG,CL40,CR and HH through assembly.By analyzing transcriptome data,all Unigenes were compared with public databases(Nr,Swiss-Prot,KOG,KEGG),and the percentage of annotated Unigenes were 65.51%,46.41%,60.57%,91.94%,54.98%in WH4,BBDG,CL40,CR and HH.Then KEGG enrichment analysis showed that there were more Unigenes in Ribosome pathway that these Unigenes were both from WH4,BBDG,CL40,and HH,and there were more Unigenes in Carbon metabolism pathway that these Unigenes were from CR.GO enrichment analysis showed that the Unigenes of WH4,BBDG,CL4O,CR and HH were all dis-tributed in three major categories:biological process,cellular component and molecular function.Combined with the enrichment analysis of KEGG,GO and KOG,the pathway map of triterpenoid saponin biosynthesis pathway in Camellia oleifera was drawn,and the key enzymes and related genes in this pathway were mined,and the differences of the related genes were analyzed.The results of this study improved the expression characteristics of genes related to the triterpenoid saponins biosynthesis pathway in Camellia oleifera,and provided scientific basis for the triterpenoid saponins biosynthesis pathway in Camellia oleifera.
作者
李杨
严和琴
于靖
郑蔚
吴友根
Li Yang;Yan Heqin;Yu Jing;Zheng Wei;Wu Yougen(Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province,School of Horticulture,Hainan University,Haikou,570228)
出处
《分子植物育种》
CAS
北大核心
2023年第11期3523-3533,共11页
Molecular Plant Breeding
基金
海南省重点研发计划项目(ZDYF2022SHFZ020)
海南省自然科学基金高层次人才项目(820RC585)共同资助。
