摘要
为探究高能电子束辐照对川产道地中药材杜仲叶品质的影响,本试验研究了不同辐照剂量(0、2、4、6、8 kGy)的高能电子束辐照对杜仲叶中的微生物含量、活性物质含量、高效液相色谱(HPLC)指纹图谱、色泽、水分和浸出物含量的影响。结果显示,电子束辐照能有效降低杜仲叶中需氧菌、霉菌和酵母菌总数;HPLC分析结果表明,不同辐照剂量对杜仲叶中的活性成分绿原酸、芦丁的影响不大,其含量变化与辐照剂量均无极显著线性相关性(P>0.01);此外,不同电子束辐照剂量处理的杜仲叶指纹图谱相似度均大于0.90;随着辐照剂量的增加,杜仲叶色泽呈现先变浅再变深的变化,主要表现为a^(*)、b^(*)值先减小后增大;各辐照剂量下杜仲叶的水分、醇溶性浸出物含量均符合2020年版《中华人民共和国药典》规定。同时,2 kGy剂量的电子束辐照能有效降低杜仲叶微生物数量,且对活性成分含量和指纹图谱无显著影响。综上,2 kGy的电子束辐照剂量既能使杜仲叶微生物数量明显降低,又能够保障其品质。本研究结果为电子束辐照技术在杜仲叶贮藏中的应用提供了一定的参考依据。
In order to investigate the effects of high-energy electron-beam irradiation on quality of Eucommia ulmoides leaves from Sichuan,samples were irradiated at different irradiation doses(0,2,4,6,8kGy).The microbial count,active ingredients,high performance liquid chromatography(HPLC)fingerprint,color,moisture and extract were measured.The result showed that the total aerobic microbial count,total yeast and mold count were significantly reduced by the electron-beam irradiation treatment(P<0.05).Chlorogenic acid and rutin measured by HPLC were not significantly affected by electron-beam irradiation,but was no significant linear correlation with the irradiation dose(P>0.01).The similarity of fingerprints of Eucommia ulmoides leaves and its extracts treated with different electron-beam irradiation doses was greater than 0.90.With the increase of irradiation dose,the Hunter color values were lighter and then darker,with the a*and b*values showing a decrease and then increase trend.The moisture and alcohol-soluble extracts are in line with the provisions of the 2020 edition of the Chinese Pharmacopoeia.Electron-beam irradiation at the dose of 2 kGy can effectively reduce the number of microbial loads,and has no significant effect on the active ingredient contents and fingerprint.In summary,the electron-beam irradiation at 2 kGy dose can significantly reduce the number of Eucommia ulmoides leaves microbial population,and ensure its quality.This study provided a reference for the application of electron-beam irradiation technology in Eucommia ulmoides leaves storage.
作者
唐艺文
陈谦
王钢
付孟
黄敏
王丹
高鹏
TANG Yiwen;CHEN Qian;WANG Gang;FU Meng;HUANG Min;WANG Dan;GAO Peng(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang,Sichuan 621010;Sichuan Institute of Atomic Energy,Chengdu,Sichuan 610101;Irradiation Preservation Key Laboratory of Sichuan Province,Chengdu,Sichuan 610101)
出处
《核农学报》
CAS
CSCD
北大核心
2023年第5期962-970,共9页
Journal of Nuclear Agricultural Sciences
基金
四川省重大科技专项项目(2019ZDZX0003)
四川省科技创新人才项目(2021JDRC0042)
四川省苗子工程项目(2021JDRC0121)
四川省区域创新合作项目(2022YFQ0044)。
关键词
电子束辐照
杜仲叶
微生物含量
指纹图谱
有效成分
electron-beam irradiation
Eucommia ulmoides leaves
microbial content
fingerprint
active ingredient
