摘要
目的利用生物信息学手段,在肾透明细胞癌(KIRC)中筛选目标基因AURKB,分析其在肾透明细胞癌中的临床意义。方法在GEO数据库下载GSE66271数据集后,筛选差异基因。基于STRING数据库和Cytoscape软件挑选hub基因。利用GEPIA在线数据库验证基因AURKB在肾透明细胞癌组织中的表达及预后关系。通过免疫组化实验验证AURKB在肾透明细胞癌组织与癌旁正常组织的表达差异。结果对数据集GSE66271分析后,共获得517个差异基因,其中155个上调基因,362个下调基因。在差异基因网络中的10个枢纽基因,从中选取基因AURKB进行验证。GEPIA数据库分析显示AURKB高表达均显著降低了KIRC患者的总生存期(OS)及无病生存期(DFS)(均P<0.001),表示其可能为KIRC的促癌基因。此外AURKB的表达与KIRC患者的不同疾病阶段呈正相关关系(P<0.001)。免疫组化实验表明AURKB蛋白表达主要在细胞核内,在癌组织中阳性表达(58.00%,29/50)高于癌旁正常组织(24.00%,12/50),差异有统计学意义(χ^(2)=11.947,P=0.001)。结论基因AURKB在肾透明细胞癌中高表达并导致了不良预后,证明其可能为KIRC的一个促癌基因,有望成为KIRC的治疗新靶点。
Objective The target gene AURKB was screened in renal clear cell carcinoma by bioinformatics method,and its clinical significance in renal clear cell carcinoma was analyzed.Methods The GSE66271 dataset was downloaded from GEO database and the differential genes were screened.Hub genes were selected based on STRING database and Cytoscape software.The expression and prognostic relationship of AURKB gene in renal clear cell carcinoma were verified by GEPIA online database.The difference of AURKB expression in clear cell carcinoma and adjacent normal tissue was verified by immunohistochemistry.Results A total of 517 differential genes were obtained after analysis of GSE66271 data set,including 155 up-regulated genes and 362 down-regulated genes.AURKB was selected from 10 hub genes in the differential gene network for verification.GEPIA database analysis showed that AURKB high expression significantly reduced the overall survival(OS)and disease-free survival(DFS)in KIRC patients(P<0.05),indicating that AURKB may be a KIRC oncogenic gene.In addition,AURKB expression was positively correlated with different disease stages in KIRC patients(P<0.05).Immunohistochemical test showed that AURKB protein was mainly expressed in the nucleus,and the positive expression in cancer tissues(58.00%,29/50)was higher than that in adjacent normal tissues(24.00%,12/50),the difference was statistically significant(χ^(2)=11.947,P=0.001).Conclusion AURKB is highly expressed in renal clear cell carcinoma and leads to poor prognosis,suggesting that AURKB may be an oncogenic genes for KIRC,and is expected to be a new therapeutic target for KIRC.
作者
李俊
陶润
王伟杰
张家俊
LI Jun;TAO Run;WANG Weijie;ZHANG Jiajun(Department of Urology,the First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui 233004,China)
出处
《中华全科医学》
2023年第6期1060-1063,共4页
Chinese Journal of General Practice
基金
安徽省高校自然科学研究重点项目(KJ2020A1290)。
