摘要
提出一种基于氧化石墨烯(GO)微纳光纤的生物传感器,将其用于狂犬病毒(RV)的免疫检测研究。首先,将标准单模光纤通过熔接机放电形成双锥形光纤,再对双锥形光纤进行熔融拉锥制作出高灵敏度的微纳光纤。然后,在微纳光纤表面修饰GO,并将RV抗原固定于该传感器表面,用于对RV抗体的特异性检测实验。实验结果表明:该生物传感器对RV抗体的检测范围为200 fg/mL~1 ng/mL,检测极限(LOD)约为225.56 fg/mL,其检测灵敏度约为1.099 nm/log(mg·mL^(−1)),解离系数约为2.92×10^(−11)M;当用于不同的抗体溶液样本和RV阳性血清的对照检测及临床检测时,该免疫传感器对前者的响应非常微弱,而对后者有明显的响应,说明其对RV抗体具有良好的特异性。基于GO修饰微纳光纤的免疫传感器具有制作简单、微纳尺寸、灵敏度高、成本低等优点。
In this study,an immunosensor based on a graphene oxide(GO)-functionalized microfiber is developed and used for the analysis of rabies virus(RV)immunodetection.For this,first,a standard single-mode fiber was discharged through an optical fiber fusion splicer to form a double-tapered fiber,after which the double-tapered fiber was fused and tapered to produce a high-sensitivity microfiber.Subsequently,the microfiber surface was modified with GO,and RV antigens were immobilized on the surface of the sensor for the immunoassay of RV antibodies.The experimental results indicate that the detection range of the biosensor for RV antibodies is 200 fg/mL^(−1)ng/mL,its detection of limit(LOD)is approximately 225.56 fg/mL,and its detection sensitivity and dissociation coefficient are approximately 1.099 nm/log(mg·mL^(−1))and 2.92×10^(−11)M,respectively.When the sensor is used in control experiments and clinical immunoassays for other antibody solution samples and RV-positive serum,it yields weak responses to the former and significant responses to the latter,indicating its high specificity to RV antibodies.The proposed immunosensor based on the GO-modified microfiber demonstrates the advantages of facile fabrication,micro-nano size,high sensitivity,and low cost.
作者
杨琪
罗彬彬
谷志鹏
吴胜昔
石胜辉
赵明富
Yang Qi;Luo Binbin;Gu Zhipeng;Wu Shengxi;Shi Shenghui;Zhao Mingfu(Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection,Chongqing University of Technology,Chongqing 400054,China;Chongqing Key Laboratory of Medicinal Chemistry and Molecular Pharmacology,Chongqing University of Technology,Chongqing 400054,China)
出处
《激光与光电子学进展》
CSCD
北大核心
2023年第7期359-366,共8页
Laser & Optoelectronics Progress
基金
国家自然科学基金(61875026)
重庆英才青年拔尖人才计划(cstc2021ycjh-bgzxm0128)
重庆市教委科学技术研究重点项目(KJZD-K202201106,KJZD-K201901101)
重庆理工大学研究生科研创新项目(clgycx20202042)。
