摘要
目的探究胃癌微环境中血小板反应蛋白4(THBS4)表达的临床病理意义及与预后的关系。方法选择2015至2018年就诊的胃癌患者100例,依据各肿瘤组织中THBS4的表达将患者分为高THBS4(n=38)组和低THBS4(n=62)组。检测胃癌相关成纤维细胞(CAFs)和胃癌细胞系中THBS 4的表达。分析THBS4的表达与胃癌患者病理特征的关系,并在随访3年记录期间患者生存情况。结果THBS4表达水平与α-平滑肌肌动蛋白水(α-SMA)平呈正相关;与肿瘤直径,高肿瘤浸润深度,淋巴结转移,临床Ⅲ、Ⅳ期,肿瘤分化程度显著相关(P<0.001),且低分化肿瘤组织中THBS4的表达明显高于高或中分化肿瘤组织(P<0.001),与患者年龄、性别及远处转移无相关性(P>0.05)。胃癌组织CAFs中α-SMA的表达与肿瘤直径,淋巴结转移显著正相关(P<0.05);与性别、年龄、及组织学分级无关(P>0.05)。高THBS4表达组3年生存率(41.03%)明显低于低THBS4表达组(64.57%),差异有统计学意义(P<0.001)。结论THBS4在胃癌微环境中表达,CAFs中的THBS4与胃癌的发展密切相关,且可以作为胃癌患者的预后评估指标。
Objective To explore the pathological significance and clinical prognosisits of thrombospondin-4(THBS4)expression in gastric cancer(GC)microenvironment.Methods From 2015 to 2018,100 patients with GC treated in our hospital were assigned into high THBS4 group(n=38)and low THBS4 group(n=62)according to THBS4 expression in tumor tissues.The expression of cancer-associated fibroblasts(CAFs)and THBS4 in gastric cancer cell lines was detected.The correlation between THBS4 expression and pathological features was analyzed,and the 3-year follow-up of survival rate was recorded.Results The expression of THBS4 was significantly positive correlated with highαsmooth muscle actin(α-SMA),and which was significantly associated with tumor diameter,deep tumor invasion depth,lymph node metastasis(LNM),clinical stageⅢandⅣ,differentiation of tumors(P<0.001).The expression of THBS4 in poorly differentiated tumor tissues was significantly higher than that in well-differentiated or moderately-differentiated tumor tissues(P<0.001),and which was not associated with age,sex and distant metastasis(P>0.05).The expression ofα-sma-positive fibroblasts(CAFs)exhibited significant correlations with tumor diameter and LNM(P<0.05),and no correlations with sex,age and histological grading(P>0.05).The 3-year survival rate in the high THBS4 group was significantly lower than that in the low THBS4 group(41.03%vs 64.57%,P<0.001).Conclusion The expression of THBS4 and CAFs in the gastric cancer microenvironment is closely associated with the development of GC,which can be used as a prognostic indicator for GC patients.
作者
刘红芬
王征
LIU Hongfen;WANG Zheng(Department of Gastroenterology,Shijiazhuang People’s Hospital,Hebei,Shijiazhuang 050021,China)
出处
《河北医药》
CAS
2023年第8期1229-1231,共3页
Hebei Medical Journal
基金
河北省医学科学研究重点课题(编号:20191481)。
关键词
血小板反应蛋白4
癌相关成纤维细胞
胃癌
癌症转移
预后
thrombospondin-4
cancer-associated fibroblasts
gastric cancer
cancer metastasis
prognosis
