摘要
Chinese cabbage has a high annual demand in China.However,clubroot disease caused by the infection of Plasmodiophora brassicae seriously affects its yield.Transcriptome analysis identified a root meristem growth factor 6(BrRGF6)as significantly up-regulated in Chinese cabbage roots infected with Plasmodiophora brassicae.Quantitative reverse-transcription polymerase chain reaction and in situ hybridization analysis showed higher BrRGF6 expression in susceptible materials than in resistant materials.After Plasmodiophora brassicae infection,BrRGF6 expression was significantly up-regulated,especially in susceptible materials.Gene function analysis showed that the roots of Arabidopsis mutant rgf6 grew faster than the wild-type,and delayed the infection progress of Plasmodiophora brassicae.A Protein,nuclear transcription factor Y subunit C(BrNF-YC),was screened from yeast two-hybrid library of Chinese cabbage induced by Plasmodiophora brassicae,and verified to interact with BrRGF6 by yeast two-hybrid co-transfer.Yeast one-hybrid andβGlucuronidase activity analysis showed that BrNF-YC could directly bind to and strongly activate the promoter of BrRGF6.Transgenic verification showed that BrRGF6 or BrNF-YC silenced Chinese cabbage significantly decreased the expression of BrRGF6,accelerated root development,and reduced incidence of clubroot disease.However,after overexpression of BrRGF6 or BrNF-YC,the phenotype showed a reverse trend.Therefore,BrRGF6 silencing accelerated root growth and enhanced resistance to clubroot disease,which was regulated by BrNF-YC.
基金
supported by grants from the National Natural Science Foundation of China[grant number 31972412,32272717].
