摘要
目的评价简易雾化设备构建的脂多糖吸入诱导小鼠急性肺炎模型。方法脂多糖雾化吸入造模后,不同时间点观察小鼠体质量与肺灌洗液中总蛋白、一氧化氮(nitric oxide,NO)、白细胞水平的变化,同时对其肺组织进行HE、Masson以及TUNEL染色病理学检测,观察模型构建后动物的恢复情况。结果与对照组比较,造模后模型小鼠体质量显著下降(P<0.01),肺灌洗液中总蛋白水平与白细胞数显著上升(P<0.01),NO水平显著降低(P<0.01)。肺部病理学检查结果表明,模型组小鼠肺组织损伤严重,并出现纤维化,且存在大量细胞凋亡,与对照组有显著差异。结论利用简易雾化设备可成功构建脂多糖诱导的小鼠急性肺炎模型,模型较为稳定,可用于急性肺炎药效的筛选。
OBJECTIVE To evaluate the mouse model of acute pneumonia induced by lipopolysaccharide inhalation constructed by simple atomization equipment.METHODS After lipopolysaccharide atomization inhalation,the changes of body weight,total protein,nitric oxide(NO)and leukocyte levels in lung lavage fluid of mice were observed at different time points.At the same time,HE,Masson and TUNEL staining were performed on the lung tissue for pathological detection.Observed the recovery of animals after model construction.RESULTS Compared with the control group,the body weight of the model mice decreased significantly(P<0.01),the total protein level and the number of white blood cells in the lung lavage fluid significantly increased(P<0.01),and the NO level significantly decreased(P<0.01)after modeling.The results of lung pathological examination showed that the lung tissue of the model group was seriously damaged,fibrosis occurred,and a large number of cells apoptosis,which was significantly different from that of the control group.CONCLUSION The mouse model of acute pneumonia induced by lipopolysaccharides can be successfully constructed by using a simple atomization.The model is relatively stable and can be used to screen the drug efficacy.
作者
业康
李振皓
蔡犇
黄晶晶
谢珍
金捷
金祖汉
余琪
王志安
YE Kang;LI Zhenhao;CAI Ben;HUANG Jingjing;XIE Zhen;JIN Jie;JIN Zuhan;YU Qi;WANG Zhi’an(Zhejiang Research Institute of Traditional Chinese Medicine Co.,Ltd.,Hangzhou 310023,China;Zhejiang Shouxiangu Botanical Drug Institute Co.,Ltd.,Hangzhou 311121,China;Zhejiang Yongning Pharmaceutical Co.,Ltd.,Taizhou 318020,China;Zhejiang Institute for Food and Drug Control,Hangzhou 310052,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2023年第7期888-893,共6页
Chinese Journal of Modern Applied Pharmacy
关键词
脂多糖
急性肺炎
雾化吸入
动物模型
lipopolysaccharides
acute pneumonia
atomization
animal model
