摘要
为筛选合适狂犬病抗体检测的试剂盒,该研究以荧光抗体病毒中和试验(fluorescent antibody virus neutralization FAVN)方法为参照,与3个ELISA试剂盒方法进行比对,进行一致性分析,并计算了三种试剂盒的敏感性和特异性。结果显示:三种不同的试剂盒中,试剂盒A与FAVN一致性最好,Kappa值为0.605,有较高的一致性,符合率为86%;试剂盒B次之,Kappa值为0.485,为中度一致,符合率为80%;试剂盒C与FAVN的一致性最差,Kappa值为0.310,符合率也最低,为74%。在敏感性和特异性方面,试剂盒A的敏感性最高,为92.1%,试剂盒B的特异性最高,为72.7%。本研究为临床上科学的筛选试剂盒提供了依据。
In order to select suitable kits for the detection of rabies antibodies,the FAVN method was used as the reference and compared with three ELISA kits for consistency analysis.The results showed that kit A had the best consistency and coincidence rate(86%)with Fluorescent Antibody Virus Neutralization(FAVN),its kappa value was 0.605;the Kappa value of kit B was 0.485,which was moderate consistent,the coincidence rate was 80%;the Kappa value of kit C was 0.310,indicating poor consistency between the results of the two reagents.The sensitivity and specificity of the three kits were calculated.Kit A had the highest sensitivity(92.1%)and Kit B had the highest specificity(72.7%).This study provides a scientific basis for screening kits in clinical practice.
作者
洪伟彬
莫钻兰
殷三鸿
叶毅飞
李敏
徐振娜
黄世品
HONG Weibin;MO Zuanlan;YIN Sanhong;YE Yifei;LI Min;XU ZhenNa;HUANG ShiPin(Dongguan Animal Disease Control and Prevention Center,Dongguan Guangdong 523086)
出处
《广东畜牧兽医科技》
2023年第2期70-73,共4页
Guangdong Journal of Animal and Veterinary Science
