摘要
ε-聚赖氨酸(ε-poly-L-lysine,ε-PL)是抑菌谱广泛的天然抑菌剂,由通过α-羧基与ε-氨基连接的25–35个赖氨酸聚合而成。ε-PL主要由白色链霉菌发酵生产所得,比化学生产更加高效和环保。ε-PL具有水溶性好、耐热和对环境无污染等特点,具有良好的应用前景。本文从发酵生产入手,着重综述了ε-PL对各种微生物抑菌性能、抑菌机制及抑菌机制模型的研究进展。推测ε-PL是通过对细胞膜的破坏而改变细胞的通透性,或者作用到细胞内引起活性氧(reactive oxygen species,ROS)胁迫而影响调节基因的表达,从而起到抑菌作用。根据这2种抑菌方式分别建立了相应的抑菌模型,即毡毯模型和ROS诱导细胞凋亡模型。本文可为ε-PL对微生物抑制性能的深入研究提供依据,同时也提出了ε-PL抑菌机制的新模型,为扩展ε-PL应用领域提供了一定的参考。
ε-poly-L-lysine(ε-PL)is a natural antibacterial agent with a wide range of bacteriostatic spectrum.It is synthesized by the polymerization of 25 to 35 lysines linked byα-carboxyl group andε-amino group.ε-PL is mainly produced by the fermentation of Streptomyces albulus,which is more efficient and environmentally friendly than chemical production.ε-PL has many excellent properties,such as good water solubility,heat resistance,edibility and nontoxicity to the environment,and has good application prospects.In this paper,the research progress ofε-PL on the bacteriostatic properties,bacteriostatic mechanisms,and bacteriostatic mechanism models of various microorganisms were reviewed.It was speculated thatε-PL may affect the expression of regulatory genes by destroying the cell membrane,changing the permeability of cells,or acting on the cells to cause the stress of reactive oxygen(ROS),so as to play an antibacterial role.According to these two bacteriostatic methods,corresponding bacteriostatic models were established,respectively,namely the carpet model and the ROS-induced apoptosis model.This study provides references for the further study ofε-PL on microbial inhibition,and also puts forward a new model ofε-PL antibacterial mechanism,thus providing certain references for expanding the application field ofε-PL.
作者
白森萌
扶教龙
王月
庄孝东
陈佳佳
王桃云
纪丽莲
BAI Senmeng;FU Jiaolong;WANG Yue;ZHUANG Xiaodong;CHEN Jiajia;WANG Taoyun;JI Lilian(School of Chemistry and Life Sciences,Suzhou University of Science and Technology,Suzhou 215009,Jiangsu,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2023年第3期1310-1323,共14页
Microbiology China
基金
国家自然科学基金(31770903)
苏州科技大学科研基金(XKZ201411)。
关键词
Ε-聚赖氨酸
天然抑菌剂
抑菌机理
抑菌模型
ε-poly-L-lysine
natural antibacterial agent
antibacterial mechanism
antibacterial model
