摘要
目的探讨淫羊藿苷对心力衰竭血清处理Dahl盐敏感大鼠骨髓源性树突状细胞(dendritic cells,DCs)的影响,为淫羊藿苷治疗心力衰竭提供实验依据。方法将50只SPF级5周龄雄性Dahl盐敏感大鼠及10只同龄雄性Dahl盐抵抗大鼠喂养至18周龄后麻醉制备心力衰竭血清与正常血清;分离Dahl盐敏感大鼠DCs,将DCs分为5组,模型组(心力衰竭血清),模型组+维生素D组(1×10^(-8)mol),模型组+淫羊藿苷高、中、低剂量组(40、20、10μmol/L),取Dahl盐抵抗大鼠DCs作为对照组(正常血清)。流式细胞仪检测6组DCs表面共刺激分子CD40、CD80、CD86、CD11b、MHC-II的表达情况;RT-PCR和Western-blot检测不同剂量淫羊藿苷对DCs维生素D受体(vitamin D receptor,VDR)、1α-羟化酶(CYP27B1)、24-羟化酶(CYP24A1)mRNA和蛋白的表达情况。结果淫羊藿苷与维生素D的治疗结果趋势一致,均可降低共刺激分子CD40、CD80、CD86及MHC-II的表达,升高CD11b的表达,显著上调VDR mRNA与CYP27B1 mRNA的表达和它们的蛋白相对表达量,下调CYP24A1 mRNA和蛋白相对表达量。结论淫羊藿苷与维生素D能够抑制DCs的分化与成熟,且淫羊藿苷与维生素D可以通过升高DCs VDR,CYP27B1的表达,降低CYP24A1的表达来有效治疗心力衰竭。
Objective To investigate the effect of icariin on the bone marrow derived dendritic cells(DCs)in Dahl salt-sensitive rats with heart failure serum,and provide experimental basis for icariin treating heart failure.Methods Fifty SPF-grade 5-week-old male Dahl salt-sensitive rats and 10 Dahl salt-resistant rats of the same age were fed to 18 weeks of age and anesthetized to prepare heart failure serum and normal serum.DCs of Dahl salt-sensitive rats were separated and divided into five groups:model group(heart failure serum),model group+vitamin D group(1×10^(-8)mol),model group+icariin high and low dose groups(40,20,10μmol/L),and the DCs of Dahl salt-resistant rats were taken out as the control group(normal serum).The expressions of co-stimulatory molecules CD40,CD80,CD86,CD11b and MHC-II on the surface of DCs in the six groups were detected by flow cytometry.RT-PCR and Western-blot were used to detect the effects of different doses of icariin on the expressions of vitamin D receptor(VDR),1α-hydroxylase(CYP27B1),24-hydroxylase(CYP24A1)mRNA and protein in DCs.Results The treatment results of icariin and vitamin D showed the same trend.Both of cariin and vitamin D reduced the expressions of co-stimulatory molecules CD40,CD80,CD86 and MHC-II and increased the expression of CD11b,significantly up-regulated the expressions of VDR mRNA and CYP27B1 mRNA and their relative protein expression,and down-regulated the relative expressions of CYP24A1 mRNA and protein.Conclusion Icariin and vitamin D can inhibit the differentiation and maturation of DCs,and icariin and vitamin D can effectively treat heart failure by increasing the expressions of VDR and CYP27B1 and decreasing the expression of CYP24A1 in DCs.
作者
赵玲婕
陈波洋
李倩
周艳
高洁
柴艺汇
陈云志
ZHAO Lingjie;CHEN Boyang;LI Qian;ZHOU Yan;GAO Jie;CHAI Yihui;CHEN Yunzhi(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,Guizhou,China;Dejiang County Hospital of Ethnic Chinese Medicine,Dejiang 565200,Guizhou,China)
出处
《中华中医药学刊》
CAS
北大核心
2023年第3期126-131,共6页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81874376)
贵州省教育厅项目(黔教合KY字〔2017〕172,〔2017〕041)。
