摘要
目的研究木紫珠Callicarpa arborea Roxb.叶化学成分,并考察其抗类风湿性关节炎活性。方法木紫珠叶提取物采用硅胶柱、Sephadex LH-20凝胶柱、MCI柱、半制备型高效液相色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。CCK-8法测定各化合物对人类风湿关节炎成纤维样滑膜细胞(HFLS-RA)的细胞毒活性。结果从中分离得到15个化合物,分别鉴定为齐墩果酸(1)、乌苏酸(2)、白桦脂酸(3)、白桦脂醇(4)、α-香树素(5)、β-香树素(6)、(+)2,6-dimethyl-6(S)hydroxyl-2-trans-2,7-octadienoic acid(7)、对羟基苯甲醛(8)、半齿泽兰素(9)、紫花牡荆素(10)、芥酸酰胺(11)、叶绿醇(12)、methylα-hydroxypalmitate(13)、亚麻酸甲酯(14)、sesamin-2,2′-diol(15)。化合物4(100、200μmol/L)、7(200μmol/L)对HFLS-RA具有细胞毒活性。结论化合物3、5~15为首次从该植物中分离得到,化合物4、7具有一定的抑制HFLS-RA细胞生长作用。
AIM To study the chemical constituents from Callicarpa arborea Roxb.leaves and to investigate their anti-rheumatoid arthritis activities.METHODS The extract from C.arborea was isolated and purified by Silica gel column,Sephadex LH-20 gel column,MCI column,semi-preparative high performance liquid chromatography,then the structures of obtained compounds were identified by physicochemical properties and spectral data.CCK-8 method was used to determine the cytotoxic activities of various compounds on human fibroblast-like synoviocytes(HFLS-RA).RESULTS Fifteen compounds were isolated and identified as leanolic acid(1),ursolic acid(2),betulinic acid(3),botulin(4),α-amyrin(5),β-amyrin(6),(+)2,6-dimethyl-6(S)hydroxyl-2-trans-2,7-octadienoic acid(7),p-hydroxybenzaldehyde(8),eupatorin(9),casticin(10),erucylamide(11),phytol(12),methylα-hydroxypalmitate(13),methyl linolenate(14),sesamin-2,2′-diol(15).Compounds 4(100,200μmol/L)and 7(200μmol/L)exhibited cytotoxic activities on HFLS-RA.CONCLUSION Compounds 3,5-15 are first isolated from this plant.Compounds 4 and 7 show certain inhibitory effects on the growth of HFLS-RA cells.
作者
黄自通
易侨祺
王红刚
HUANG Zi-tong;YI Qiao-qi;WANG Hong-gang(Qingyuan Polytechnic,Qingyuan 511510,China;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China)
出处
《中成药》
CAS
CSCD
北大核心
2023年第4期1150-1155,共6页
Chinese Traditional Patent Medicine
基金
广东省高水平专业群建设项目(490201)。
关键词
木紫珠
叶
化学成分
分离鉴定
抗类风湿性关节炎活性
Callicarpa arborea Roxb.
leaves
chemical constituents
isolation and identification
anti-rheumatoid arthritis activities
