摘要
目的:研究不同时期应用复方肝素钠尿囊素凝胶对深Ⅱ度烧伤大鼠增生性瘢痕(Hypertrophic scar,HS)的影响,以期发现复方肝素钠尿囊素凝胶抑制瘢痕的最佳时间。方法:使用砝码法制备深Ⅱ度烧伤大鼠模型。根据复方肝素钠尿囊素凝胶应用时间分为14 d组(A组)、28 d组(B组)和42 d组(C组),每组根据有无应用复方肝素钠尿囊素凝胶分为未应用组(A1组、B1组、C1组)和应用组(A2组、B2组、C2组),并于应用28 d后取材,每组应用前取材作为应用前对照组(A0组、B0组、C0组)。共造模45只,每小组5只。每天固定时间均匀涂抹复方肝素钠尿囊素凝胶于各创面。对A组创面拍照并计算创面愈合率,HE染色检测各组瘢痕组织的厚度,Masson染色检测各组瘢痕组织胶原纤维的表达,RT-PCR检测各组瘢痕组织炎症因子IL-6和TNF-α mRNA的表达。结果:应用复方肝素钠尿囊素凝对创面愈合率有一定影响。A2、B2组瘢痕组织的厚度增长量较A1、B1组低,未应用组和应用组瘢痕组织厚度的差值A组、B组均大于C组,差异有统计学意义(P<0.05)。A2、B2、C2组胶原纤维光密度值的增长量较A1、B1、C1组低,未应用组和应用组胶原纤维光密度值变化量的差值比较差异有统计学意义(P<0.05);其中A组>C组。A2组IL-6和TNF-α mRNA相对表达的变化量较A1组低,B2组IL-6mRNA相对表达的变化量较B1组低,未应用组和应用组IL-6 mRNA相对表达变化量的差值A组>B组>C组,差异有统计学意义(P<0.05)。A2、B2和C2组分别较A1、B1和C1组成纤维细胞增生相对较少,胶原纤维排布相对规则。结论:深Ⅱ度烧伤后14 d、28 d和42 d应用复方肝素钠尿囊素凝胶均可一定程度的抑制大鼠瘢痕增生和胶原纤维的表达,减少炎症因子基因的表达,其中14 d应用效果最佳。
Objective To explore the effects effects of compound heparin sodium and allantoin gel at different stages on hypertrophic scar formation after deep second degree burn in rats, in order to find the best time for compound heparin sodium and allantoin gel to inhibit scars. Methods The deep second degree burn rat models were prepared by weight method.According to the application time of compound heparin sodium and allantoin gel, all rats were divided into 14 d group(group A), 28 d group(group B) and 42 d group(group C).Each group was divided into non-application groups(group A1, B1, C1)and application groups(group A2, B2, C2) according to whether the compound heparin sodium and allantoin gel was applied or not, and the samples were collected at 28 days after application. The samples collected before application were used as control groups(group A0, B0, C0). A total of 45 models were made, 5 in each group. Compound heparin sodium and allantoin gel was evenly applied to each wound at a fixed time every day. The wounds of group A were photographed and calculated the wound healing rate. The thickness of scar tissue was measured by HE staining. The expression of collagen fibers were measured by Masson staining. The inflammatory factors IL-6 and TNF-α mRNA expressions were detected by RT-PCR. Results The application of compound heparin sodium and allantoin gel had a certain effect on the wound healing rate. The increase of scar tissue thickness in groups A2 and B2 were lower than that in groups A1 and B1, respectively. The difference of scar tissue thickness between the non-application group and the application group in group A and B was greater than that in group C,the differences were statistically significant(P<0.05). The increase of collagen fiber optical density in group A2, B2 and C2 were lower than that in group A1, B1 and C1. The differences in the change of collagen fiber optical density between the nonapplication group and the application group were statistically significant(P<0.05). Among them, group A>group C. The relative expression changes of IL-6 and TNF-α mRNA in group A2 were lower than those in group A1. The relative expression of IL-6 mRNA in group B2 was lower than that in group B1. The difference of IL-6 mRNA relative expression between the non-application group and the application group was group A>group B>group C, the differences were statistically significant(P <0.05). Compared with group A1, B1 and C1, group A2, B2 and C2 constituted less fibroblasts proliferation, and the arrangement of collagen fibers were relatively regular. Conclusion Application of compound heparin sodium and allantoin gel on 14 d, 28 d, and 42 d after deep second degree burns can inhibit hypertrophic scars in rats to a certain extent, inhibit the expression of collagen fibers, and reduce the expression of inflammatory factor genes. 14 d was the best choice.
作者
邢亮
冯建科
马磊磊
魏伟
张庆富
张涵
XING Liang;FENG Jianke;MA Leilei;WEI Wei;ZHANG Qingfu;ZHANG Han(Department of Burns and Plastic Surgery,the First Hospital of Hebei Medical University,Shijiazhuang 050000,Hebei,China)
出处
《中国美容医学》
CAS
2023年第2期9-14,共6页
Chinese Journal of Aesthetic Medicine
基金
河北省政府资助专科带头人培养项目(编号:LS201805)
河北省卫生健康委员会基金项目(编号:20200116)。
关键词
复方肝素钠尿囊素凝胶
烧伤
深Ⅱ度
增生性瘢痕
胶原纤维
炎症因子
compound heparin sodium and allantoin gel
burns
deep second degree
hypertrophic scar
collagen fibers
inflammatory cytokines
