摘要
本试验旨在研究丁酸梭菌和枯草芽孢杆菌配伍对肉鸡生长性能、血清生化指标及盲肠微生物区系的影响。试验选用1日龄罗斯308肉鸡600只,随机分为2组,每组6个重复,每个重复50只鸡。对照组饲喂基础饲粮,试验组饲喂在基础饲粮中同时添加2×108CFU/kg丁酸梭菌和1×109CFU/kg枯草芽孢杆菌的试验饲粮,试验期为42 d。结果表明:1)与对照组相比,饲粮中同时添加丁酸梭菌和枯草芽孢杆菌显著提高了42日龄肉鸡的平均日采食量和平均日增重(P<0.05),显著降低了42日龄肉鸡的料重比(P<0.05)。2)与对照组相比,丁酸梭菌和枯草芽孢杆菌配伍组42日龄肉鸡肝脏中的甘油三酯含量显著降低(P<0.05)。3)与对照组相比,饲粮中同时添加丁酸梭菌和枯草芽孢杆菌在门水平上显著提高了42日龄肉鸡盲肠中的厚壁菌门(Firmicutes)和放线菌门(Actinobacteriota)的相对丰度(P<0.05),降低了42日龄肉鸡盲肠中的拟杆菌门(Bacteroidetes)、弯曲杆菌门(Campilobacterota)、脱硫菌门(Desulfobacterota)和蓝藻门(Cyanobacteria)的相对丰度(P<0.05);在属水平上显著提高了42日龄肉鸡盲肠中的双歧杆菌属(Bifidobacterium)和产粪甾醇真细菌群(Eubacterium_coprostanoligenes_group)的相对丰度(P<0.05),降低了42日龄肉鸡盲肠中的螺杆菌属(Helicobacter)和副拟杆菌属(Parabacteroides)的相对丰度(P<0.05);且厚壁菌门、放线菌门、CHKCI001、双歧杆菌属、产粪甾醇真细菌群的相对丰富与肉鸡的生长性能呈正相关关系(P<0.05),拟杆菌门、弯曲杆菌门、脱硫菌门、蓝藻门、拟杆菌属、螺杆菌属、副拟杆菌属的相对丰度与肉鸡的生长性能呈负相关关系(P<0.05)。由此可见,丁酸梭菌和枯草芽孢杆菌配伍具有改变42日龄肉鸡盲肠微生物区系,提高42日龄肉鸡生长性能的作用。
To investigate the effects of compatibility of Clostridium butyricum and Bacillus subtilis on growth performance,serum biochemical indices and cecal microflora of broilers,six hundred 1-day-old Ross 308 broiler chicks were randomly allocated into 2 groups with 6 replicates of 50 broilers per replicate in a completely randomized design.Birds in the control group were fed a basal diet,and those in the test group were fed the basal diet supplemented with 2×10~8 CFU/kg of C.butyricum and 1×10~9 CFU/kg of B.subtilis.The experiment lasted for 42 days.Results showed as follows:1)compared with the control group,the supplementation of C.butyricum and B.subtilis significantly increased the average daily feed intake(ADFI)and average daily gain(ADG)of broilers at 42 days of age(P<0.05),and decreased the feed/gain(F/G)of broilers at 42 days of age(P<0.05).2)Compared with the control group,the triglyceride(TG)content in the liver of broilers at 42 days of age in compatibilty of C.butyricum and B.subtilis group decreased significantly(P<0.05).3)Compared with the control group,the supplementation of C.butyricum and B.subtilis significantly increased the relative abundance of Firmicutes and Actinobacteriota in the cecum of broilers at 42 days of age(P<0.05),and decreased the relative abundance of Bacteroidetes,Campilobacterota,Desulfobacterota and Cyanobacteria in the cecum of broilers at 42 days of age at phylum level(P<0.05).The supplementation of C.butyricum and B.subtilis significantly increased the relative abundance of Bifidobacterium and Eubacterium_coprostanoligenes_group in the cecum of broilers at 42 days of age(P<0.05),and decreased the relative abundance of Helicobacter and Parabacteroides in the cecum of broilers at 42 days of age at genus level(P<0.05).The relative abundance of Firmicutes,Actinobacteriota,CHKCI001,Bifidobacterium,Eubacterium_coprostanoligenes_group was positively correlated with the growth performance(P<0.05)and the relative abundance of Bacteroidetes,Campilobacterota,Desulfobacterota,Cyanobacteria,Bacteroides,Helicobacter,Parabacteroides was negatively correlated with the growth performance of broilers(P<0.05).In conclusion,dietary supplementation with C.butyricum and B.subtilis can change the cecal microflora and improve the growth performance of broilers at 42 days of age.
作者
赵旭
庄佳荣
李洪涛
于娟
张金红
侯彦茹
ZHAO Xu;ZHUANG Jiarong;LI Hongtao;YU Juan;ZHANG Jinhong;HOU Yanru(College of Agriculture and Forestry Science,Linyi University,Linyi 276000,China;Shandong Backbone Feed Group Co.,Ltd.,Linyi 276000,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2023年第2期845-854,共10页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
山东省自然科学基金面上项目(ZR2021MC170)
临沂大学高层次人才科研启动项目(LYDX2019BS036)。
