摘要
以具有6个连续节间的沟叶结缕草的克隆片段为实验材料,母株正常浇水,子株分别种在正常含水量的100%、60%、40%和20%的水分条件下,匍匐茎被切断或连接,处理14 d后通过测定子株的生理和生长指标来研究沟叶结缕草的生理整合方向和特点,以期从群体上揭示沟叶结缕草的抗旱机理。结果表明,克隆整合显著降低了水分异质条件下连接子株的超氧阴离子产率、H_(2)O_(2)含量、丙二醛含量和相对电导率,其连接子株的过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPX)和超氧化物歧化酶(SOD)的活性也显著低于断开子株,但匍匐茎连接状态对过氧化物酶(POD)活性没有显著影响。克隆整合促进了子株和克隆片段的生长,子株的根冠比也发生了显著变化,但母株的生长没有受到显著影响。综上所述,在水分异质生境下,沟叶结缕草存在着母株对子株的生理整合作用,有助于缓解干旱胁迫对子株的不利影响。
In the study,clonal fragments with six consecutive internodes from stoloniferous Zoysia matrella were used as experimental materials.The daughter ramets were grown in different soil water conditions(100%,60%,40%,or 20% of normal condition),while the parent ones were watered under normal condition.Stolons were severed or retained intact.The physiological integration direction and characteristics of Z.matrella were studied by measuring the physiological and growth indexes of daughter plants after 14 d treatment,with the aim to reveal the drought resistance mechanisms of Z.matrella at the population level.The results showed that clonal integration significantly reduced O_(2)^(-)·production rate,H_(2)O_(2)content,MDA content and relative conductivity of daughter ramets with connected stolons subjected to water heterogeneous habit when compared with daughter plants with severed stolons.Activities of Catalase(CAT),glutathione peroxidase(GPX)and superoxide dismutase(SOD)of daughter plants with connected stolons were significantly lower than those with severed stolons.However,the peroxidase(POD)activity of daughter ramets was not affected.Clonal integration benefited clonal fragments and daughter ramets growth,but not did not affect parent ramets growth.The root to shoot ratio of daughter plant were also changed significantly.These results indicate that a physiological integration existed from parent ramets to daughter ramets,and the latter benefited from parent ramets under water heterogeneous habit,which was important for Z.matrella adaption to water stress.
作者
蔡烨琳
景欣悦
朱丁香
胡化广
张振铭
CAI Ye-lin;JING Xin-yue;ZHU Ding-xiang;HU Hua-guang;ZHANG Zhen-ming(School of Marine and Bioengineering,Yancheng Teachers University,Yancheng 224007,Jiangsu Province,China)
出处
《节水灌溉》
北大核心
2023年第2期89-95,共7页
Water Saving Irrigation
基金
江苏省大学生创新创业计划训练项目(202010324054Y)资助。
关键词
水分异质性
沟叶结缕草
生理
生长
克隆整合
water heterogeneity
Zoysia matrella
physiology
growth
clonal integration
