摘要
皮肤糖化反应是导致肤色暗黄、色斑、老化的一个重要因素,针对皮肤糖化问题,开发了一种生物活性多肽并进行护肤功效验证。AIMP1衍生肽(AdP)是由氨基酰tRNA合成酶相互作用多功能蛋白质1(AIMP1)衍生而来的寡肽,具有类AIMP1的多种生物活性。采用牛血清白蛋白(BSA)糖化和羰基化体系研究AdP的抗糖化效果,并对含AdP的样品进行了临床功效测试。结果表明:AdP可减缓BSA与葡萄糖的糖基化反应,减少蛋白质羰基的生成,具有浓度依赖性;皮肤角质层含水量显著提高27.32%,TEWL降低12.77%,R2值提高10.34%,ITA°值提高14.25%,皱纹面积减少12.14%,AdP可有效抑制蛋白糖化,具有提升屏障功能、祛黄提亮抗皱的功效。
Advanced glycation endproducts(AGEs) are produced during protein glycation and associated with skin ageing.The AIMP1 derived peptide(AdP) which is derived from aminoacyl tRNA synthase interacting multifunctional protein 1 may be used as an inhibitor of glycation.This study evaluated the potential anti-glycation effect of AdP and its application in cosmetic.Thirty valid subjects were selected as the research subjects,and non-invasive instruments were used to detect and record the water content,TEWL,R2,ITA°,wrinkle area/AOI area in the test group and the control group at 0,4 and 8 weeks using corresponding products.The results show that the glycosylation reaction between BSA and glucose is inhibited by AdP,and the formation of protein carbonyl is reduced in a concentrationdependent manner.The applications of AdP in cosmetic has significant effects on improving the stratum corneum water content,the R2 value,the ITA° value,and the reducing the TEWL value and wrinkles.These findings suggest that AdP may serve as an inhibitor of glycation and provide new insights into its application.
作者
杨秀芬
马文君
李磊
王培培
郑春阳
Xiufen Yang;Wenjun Ma;Lei Li;Peipei Wang;Chunyang Zheng(Robustnique Corporation Ltd.,Tianjin 300384,China;Integribiotech Corporation Ltd.,Tianjin 300384,China)
出处
《日用化学工业（中英文）》
CAS
北大核心
2023年第2期171-179,共9页
China Surfactant Detergent & Cosmetics
基金
2021年天津市生物医药科技重大专项(21ZXSYSY00010)。
关键词
AIMP1衍生肽
抗糖化
功效研究
AIMP1 derived peptide
anti-glycosylation
efficacy study
