摘要
土壤盐渍化是影响小麦生长的重要非生物胁迫之一,筛选培育耐盐小麦种质资源对开展盐碱地综合利用具有重要意义。本研究以19份杂交小麦和2份常规品种为试验材料,以蛭石为培养基质,设置NaCl含量分别为0、0.4%、0.8%、1.2%、1.6%、2.0%的6个处理,从播种时开始盐胁迫处理,分析测定生长相关的11项指标。采用多元统计分析方法对小麦种质资源进行苗期耐盐性评价,结果表明,在1.2%盐处理下,小麦种质资源大多数指标的耐盐系数四分位差最大,因此,1.2%盐被认为是耐盐鉴定最适浓度;利用主成分分析方法可将11项调查指标的耐盐系数简化为3个主成分;通过主成分贡献率和隶属函数分析进一步将3个主成分值简化成综合评价指标D值;根据D值利用聚类分析将21份小麦种质资源划分为5类,对应高耐、耐盐、中耐、敏感和高敏5个耐盐级别,苗期耐盐鉴定表明13份杂交小麦的综合评价D值高于捷麦19与济麦22;结合逐步回归分析获得11个调查指标耐盐系数与D值的最优回归方程:D=–0.743+0.779×PLL+0.372×TNL+1.273×PH+0.336×PLC+0.279×RL+0.558×RDW,由此回归方程可知倒二叶叶长(PLL)、总叶片数(TNL)、株高(PH)、倒二叶叶绿素(PLC)、根长(RL)和根干重(RDW)可作为1.2%持续盐胁迫下小麦种质资源苗期鉴定评价指标。
Soil salinization is one of the important abiotic stresses which affects wheat growth.The screening and breeding salt-tolerant wheat is of great significance to the utilization of salinized land.19 hybrid wheat resources and 2 conventional varieties were used as the experimental materials,seeds were grown in vermiculite,6 treatments of NaCl solutions with 0,0.4%,0.8%,1.2%,1.6%,and 2.0%from sowing were set,and 11 indicators related to the growth were analyzed and determined.The multivariate statistical analysis method was used to evaluate the salt-tolerant of wheat germplasm resources at seedling stage.The results showed that the saline tolerance coefficient of most indicators were the highest interquartile range under 1.2%salinity among germplasm resources,thus the 1.2%salinity was considered as the optimum concentration for identification saline tolerance.11 indicators could be simplified into 3 principal components by using principal component analysis.Through the analysis of principal component contribution rate and membership function,the 3 principal component values were further reduced to D-value of comprehensive evaluation index.According to the D-value,21 wheat germplasm resources were divided into 5 salt-tolerant levels(high tolerance,salt tolerance,medium tolerance,sensitive and high sensitivity)by cluster analysis.The comprehensive evaluation D-values of 13 hybrid wheat varieties were higher than that of Jiemai 19 and Jimai 22 at seedling stage.Combined with stepwise regression analysis,the optimal regression equation of salt-tolerant coefficient and D-value of 11 indicators was obtained:D=–0.743+0.779×PLL+0.372×TNL+1.273×PH+0.336×PLC+0.279×RL+0.558×RDW.Based on the above regression equation,PLL(penultimate leaf length),PH(plant height),PLC(penultimate leaf chlorophyll),RL(root length),and RDW(root dry weight),could be used as evaluation indicators for wheat germplasm resources identification under 1.2%continuous salt stress at seedling stage.
作者
孙现军
姜奇彦
胡正
李宏博
庞斌双
张风廷
张胜全
张辉
SUN Xian-Jun;JIANG Qi-Yan;HU Zheng;LI Hong-Bo;PANG Bin-Shuang;ZHANG Feng-Ting;ZHANG Sheng-Quan;ZHANG Hui(Center for Crop Germplasm Resources,Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Beijing Academy of Agriculture and Forestry Science Research Institute of Hybrid Wheat,Beijing 100097,China)
出处
《作物学报》
CAS
CSCD
北大核心
2023年第4期1132-1139,共8页
Acta Agronomica Sinica
基金
中国农业科学院科技创新工程(CAAS-ZDRW202201)
北京市农林科学院科技创新能力建设专项(KJCX20210439)
国家自然科学基金项目(31601329)资助。
关键词
小麦种质资源
苗期耐盐
综合评价
wheat germplasm resources
salt-tolerant at seedling stage
comprehensive evaluation
