摘要
以干燥覆盆子粉末为原料,总黄酮提取率为考察指标,优化微波辅助低共熔溶剂对覆盆子总黄酮的提取工艺,筛选低共熔溶剂的最优组合,并考察低共熔溶剂的摩尔比、含水量、液固比、微波功率、辐射时间、回流提取时间对提取效果的影响;星点-效面法优化提取工艺。通过测定纯化后的覆盆子总黄酮对DPPH、羟基自由基的清除率考察其抗氧化活性,测定对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌生长的影响考察其抑菌活性。结果表明:氯化胆碱/山梨醇的提取率较好,单因素试验结果表明低共熔溶剂的含水量、液固比、微波功率是影响总黄酮提取的主要因素,星点-效面法优化得到的最佳提取工艺为:氯化胆碱/山梨醇摩尔比为1∶2,含水量为31%、液固比为38 mL/g,微波功率为450 W,辐射时间为150 s,提取时间为60min,在该条件下总黄酮提取率为5.88%,与预测值相对误差为1.85%;抗氧化研究结果显示纯化后的黄酮具有良好的抗氧化活性,且与药物浓度呈量效关系,抑菌活性结果显示黄酮对三种常见菌均有一定的抑制作用。本研究采用微波辅助低共熔溶剂提取覆盆子总黄酮成分的效果较好,且提取纯化后的黄酮能够保持良好的抗氧化与抑菌活性。
Having raspberry dry powder as raw material,the flavonoid extraction yield as examining index,the microwave assisted extraction process of raspberry flavonoid with deep eutectic solvent was optimized,the optimal combination of deep eutectic solvents was screened,the effects of low eutectic solvent mole ratio,water content,liquid to solid ratio,microwave power,radiation time and reflux extraction time on the extraction effect were investigated. The extraction process was optimized by star-point response surface. The antioxidant activity of purified flavonoids was evaluated by determining the scavenging rate of DPPH and hydroxyl radical,and the effects on the growth of Escherichia coli,Staphylococcus aureus and Bacillus subtilis were investigated. The results showed that the extraction efficiency of choline chloride/sorbitol was better. The single factor test results showed that water content of deep eutectic solvents,liquid-solid ratio and microwave power were the main factors affecting the extraction of flavonoids. The optimal extraction process obtained by star-point response surface was as follows :Under the conditions of 1∶2 choline chloride/sorbitol molar ratio,31% water content,38 mL/g liquid to solid ratio,450 W microwave power,150 s radiation time and 60 min extraction time,the extraction rate of flavonoids was 5.88%,and the relative error was 1.85% compared with predicted value. Antioxidant study results showed that the purified flavonoids had good antioxidant activity,and showed a dose-effect relationship with drug concentration. The results of antibacterial activity showed that flavonoids had certain inhibitory effect on the three common bacteria. In this study,microwave-assisted deep eutectic solvent extraction of flavonoids from raspberry had a good effect,and the extracted and purified flavonoids maintained good antioxidant and antibacterial activities.
作者
于秋菊
耿凤英
张磊磊
YU Qiuju;GENG Fengying;ZHANG Leilei(Xuzhou Vocational College of Bioengineering,College of Medicine and Food,Xuzhou 221006)
出处
《中国食品添加剂》
CAS
北大核心
2023年第2期43-51,共9页
China Food Additives
基金
江苏省高职院校青年教师企业实践培训项目资助(2021QYSJ083)。
关键词
覆盆子总黄酮
微波辅助
低共熔溶剂
星点-效应面法
抗氧化
抑菌
raspberry flavonoids
microwave assisted
deep eutectic solvents
star-point response surface
antioxidant
bacteriostatic
