摘要
Super-resolution optical microscopy,sometimes called optical nanoscopy,refers to a new kind of far-¯eld optical microscopy which allows optical imaging with a resolution higher than the well-known resolution limit(or the Abbe di®raction limit)which stood for more than a century.The reported approaches that break the resolution limit involve either e®ectively reducing the focus region of the excitation light or stochastic separation and precise localization of single°uorophores.Super-resolution optical microscopy provides unprecedented opportunities for tackling outstanding fundamental questions in life science,medicine,materials,and many others that require non-invasive imaging with molecular level spatial resolution.
