摘要
目的通过对不同产地的麦冬类药材及其混淆品进行系统的生药学研究,为不同产地的麦冬Ophiopogon japonicus及其混淆品的鉴定提供科学依据。方法采用性状鉴别法、常规显微鉴别法及偏振光显微鉴别法进行对比研究,结合大型图像拼接及实时景深扩展技术获取全息彩色影像数据,对麦冬的2种道地药材川麦冬、浙麦冬;山麦冬2个基原品种短葶山麦冬Liriope muscari,湖北麦冬L.spicata var.prolifera;及其他常见麦冬类药材混淆品种阔叶山麦冬L.platyphylla、金边阔叶麦冬L.platyphylla var.variegata共32批次药材进行系统的性状及显微鉴别研究。结果性状鉴别从麦冬类药材及其混淆品外观、质地、长度/直径比例及横断面能将基本区分6种麦冬类药材及其混淆品;横断面显微首次获取全息彩色影像数据,木化部位及草酸钙针晶在偏振光下具有清晰可辨的识别度,麦冬类药材及其混淆品中柱部分中柱比(中柱直径与横断面直径的比值)、石细胞层数、韧皮部束个数、木质部导管层数差异较大;粉末显微草酸钙结晶长度、石细胞璧厚度及内皮层直径具有微小差异。结论该方法简便易行,能快速鉴别麦冬类药材及其混淆品种。
Objective To provide theoretical basis of pharmacognosy for utilization and development of Maidong(Ophiopogonis Radix) produced from two places and its adulterant,and provide guidance for follow-up study.Methods The character identification and microscopic identification under normal light and polarized light were performed to systematically study on 32 batches of Ophiopogonis Radix and its adulterants,including Ophiopogon japonicus from Sichuan and Zhejiang;Liriope muscari,Liriope spicatavar.prolifera,Liriope platyphylla and Liriope platyphylla var.variegata.Using big graph image merging and real-time depth of field expansion photography,the data of holographic color microscopic image were obtained.Results Six kinds of Ophiopogonix Radix and its adulterants can be basically distinguished from character identification of the appearance,ratio of length to diameter,texture and cross-section.It’s the first time to obtain holographic color image data by cross-sectional microscopy,wood parts and calcium oxalate needle crystal in polarized light had a clear discernibility.There were significant differences in the ratio of stele(the ratio of the diameter of the stele to the diameter of the cross section),the number of stone cell layers,the number of phloem bundles,and the number of xylem vessels in Ophiopogonis Radix and its adulterants.There were slight differences in the length of calcium oxalate crystal,wall thickness of stone cells and the diameter of the endodermis.Conclusion This method is simple and feasible,and can quickly identify Ophiopogonis Radix and its adulterants.
作者
蒙倩
张航
Chayanis Sutcharitchan
杨纯国
吴婉莹
李泳锋
崔亚君
MENG Qian;ZHANG Hang;Chayanis Sutcharitchan;YANG Chun-guo;WU Wan-ying;LI Yong-feng;CUI Ya-jun(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shandong Yifang Pharmaceutical Co.,Ltd.,Linyi 276067,China;Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201210,China;Hubei Chuanchuang Chinese Medicinal Materials Technology Co.,Ltd.,Enshi 445600,China)
出处
《中草药》
CAS
CSCD
北大核心
2022年第24期7905-7914,共10页
Chinese Traditional and Herbal Drugs
基金
国家重点研发计划“中医药现代化研究”重点专项项目(2018YFC1707902)。
关键词
麦冬
山麦冬
景深扩展
大图拼接
偏振光
性状鉴别
显微鉴别
Ophiopogonis Radix
Liriopes Radix
field expansion photography
big graph image merging
polarized light
morphological identification
microscopic identification
