摘要
利用高山杜鹃优异品种与野生种质进行杂交是培育自主观叶新品种的有效途径。本研究开展以高山杜鹃‘XXL’(Rhododendron’XXL’)为母本,蓝果杜鹃(R.cyanocarpum)为父本进行杂交授粉,用荧光显微镜观察授粉花粉萌发及花粉管的生长过程,调查其子房膨大率和结实率。结果显示,在授粉后1~8 d,蓝果杜鹃的花粉萌发率呈缓慢上升趋势,‘XXL’花粉管在花柱中呈显著伸长趋势,期间无受精情况发生;在授粉后11~17 d,花粉管进入‘XXL’子房并发生受精;在授粉后17 d,其花粉萌发率和受精胚珠数达最大值,分别为(31.67±8.50)%和(13.67±3.79)个。在花粉管生长过程中,在柱头细胞、花粉管、花柱、子房和胚珠等多处出现异常胼胝质沉积反应;其杂交子房膨大率为18.75%,结籽率为零。研究表明,高山杜鹃‘XXL’与蓝果杜鹃的杂交不亲和性明显且可能存在较强的杂交障碍。
It is the effective method to breed the independent new varieries by the crosses between the excellent varieties and the wild species.Female Rhododendron’XXL’was used for pollination with male R.cyanocarpum in this study.The process of pollen germination and pollen tube growth were observed by fluorescence microscope,and the ovary enlargement rate and seed setting rate were investigated.The results showed that the pollen germination rate of R.cyanocarpum showed a slow upward trend and no fertilization happened at 1~8 days after pollination.And the pollen tubes entered the’XXL’ovary and then the fertilization occurred at 11~17 days after pollination.At 17 days after pollination,the pollen germination rate and the number of fertilized ovules reached the maximum,which were(31.67±8.50)%and(13.67±3.79)respectively;during the pollen tube growth,it was companied with a series of callose deposited responses,which was happened in the stigma cell,pollen tube,canal cell,ovary and ovules;the ovary-swelling rate was 18.75%and no seed set in the cross.The results indicated that the cross between Rhododendron’XXL’and R.cyanocarpum was incompatible and its fertilization barriers might be strong.
作者
解玮佳
宋杰
唐路瑶
彭绿春
李世峰
王继华
Xie Weijia;Song Jie;Tang Luyao;Peng Lüchun;Li Shifeng;Wang Jihua(National Engineering Research Center for Ornamental Horticulture,Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming,650205;School of Life Sciences,Yunnan University,Kunming,650091)
出处
《分子植物育种》
CAS
北大核心
2023年第2期582-588,共7页
Molecular Plant Breeding
基金
国家自然科学基金地区科学基金项目(31760231)
云南省科技厅重大科技专项(2019ZG006,202002AA10-0007)
云南省技术创新人才培养配套项目(2018-HB082)共同资助。
