摘要
以白三叶(Trifolium repens L.‘Haifa’)为研究对象,研究了0.8 mmol·L-1胺鲜酯(DA-6)浸种处理对白三叶种子在盐胁迫条件下萌发的影响,并对DA-6诱导的耐盐机理进行初步研究,为白三叶的栽培管理提供参考。结果表明,DA-6浸种显著提高了盐胁迫下种子的发芽势、发芽率、活力指数、发芽指数、茎长和鲜干重(P<0.05)。盐胁迫显著诱导了游离脯氨酸和可溶性糖的积累,降低了细胞渗透势(P<0.05)。此外,盐胁迫导致了萌发种子中活性氧(超氧阴离子和过氧化氢)和膜脂过氧化产物丙二醛含量显著增加,电导率显著升高(P<0.05)。DA-6浸种前处理显著提高了胁迫下抗氧化酶(SOD、POD和APX)的活性,增幅分别为33.0%、31.9%和49.1%,这有效地缓解了由盐胁迫引起的氧化损伤,有利于幼苗稳定细胞膜(P<0.05)。本研究表明DA-6浸种处理通过增强抗氧化防御并降低细胞氧化伤害,从而提高盐胁迫下白三叶种子的萌发特性和胁迫耐受性。
Effects of 0.8 mmol·L-1 diethyl aminoethyl hexanote(DA-6)treatment on seeds germination of white clover(Trifolium repens L.‘Haifa’)were investigated under salt stress.One hundred mmol·L-1 NaCl was used as a salt stress treatment to provide a reference for the cultivation and management of turfgrass in urban greenery.Seeds soaking with DA-6 significantly increased the germination vigor,germination percentage,germination index,vitality index,stem length and fresh/dry weight of seedlings(P<0.05).Salt stress significantly induced the accumulation of free proline and soluble sugar,followed by a significantly lower cellular osmotic potential(P<0.05).However,pretreatment with DA-6 immersion under salt stress had no significant effect on the accumulation of free proline and soluble sugar in seedlings(P>0.05).In addition,salt stress led to a significant increase in the content of reactive oxygen species(superoxide anion and hydrogen peroxide),a membrane lipid peroxidation product malondialdehyde,and electrolyte leakage in seedlings(P<0.05).Our current study indicated that DA-6 soaking improved germination characteristics and stress tolerance of white clover seeds by enhancing antioxidant defense and reducing cellular oxidative damage under salt stress.
作者
曹亦芹
程碧真
李州
CAO Yi-qin;CHEN Bi-zhen;LI Zhou(College of Grassland Science,Sichuan Agricultural University,Chengdu,Sichuan Province,611130,China)
出处
《草地学报》
CAS
CSCD
北大核心
2023年第1期140-147,共8页
Acta Agrestia Sinica
基金
四川省科技创新(苗子工程)培育项目(2021064)资助。
关键词
白三叶
DA-6
盐胁迫
渗透调节
抗氧化防御
White clover
Diethyl aminoethyl hexanote
Salt stress
Osmoregulation
Antioxidant defense
