摘要
目的:探究鱼腥草对葡聚糖硫酸钠(dextran sulfate sodium,DSS)诱导的溃疡性结肠炎(ulcerative colitis,UC)小鼠炎症及病理损伤的缓解作用。方法:将72只雄性C57BL/6J小鼠随机分成空白组、模型组、阳性组以及鱼腥草汁低、中、高剂量组。除空白组外,其他组采用质量分数3%DSS溶液建立UC小鼠模型,造模后第4天开始灌胃给药,连续给药10 d,计算小鼠疾病活动指数(disease activity index,DAI)评分,测定小鼠结肠组织中丙二醛(malondialdehyde,MDA)含量,肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、干扰素γ(interferon-γ,IFN-γ)、白细胞介素(interleukin,IL)-1β、IL-4、IL-6、IL-10质量浓度,髓过氧化物酶(myeloperoxidase,MPO)、超氧化物歧化酶(superoxide dismutase,SOD)活力,血清脂多糖(lipopolysaccharides,LPS)水平、D-乳酸(D-lactic acid,D-La)浓度,并利用苏木精-伊红染色法观察结肠组织病理学变化。结果:与模型组相比,鱼腥草汁低、中、高剂量组整体便血情况均有所好转,精神状态较佳,中、高剂量组小鼠体质量、结肠长度极显著增加(P<0.01),胸腺指数极显著升高(P<0.01),DAI评分、脾脏指数极显著降低(P<0.01),TNF-α、IL-1β、IL-6、IFN-γ质量浓度极显著降低(P<0.01),IL-4、IL-10质量浓度极显著升高(P<0.01),MDA含量及MPO活力极显著降低(P<0.01),SOD活力极显著升高(P<0.01),血清D-La浓度和LPS水平极显著降低(P<0.01),结肠组织损伤明显减轻,病理学评分极显著降低(P<0.01)。结论:鱼腥草汁可改善DSS诱导的UC小鼠的炎症反应,降低结肠炎小鼠肠道通透性,能调节小鼠体内的氧化应激水平及缓解肠道损伤。
The purpose of this study was to investigate the effect of Houttuynia cordata on inflammation and pathological injury in mice with ulcerative colitis(UC)induced by dextran sulfate sodium(DSS).Seventy-two male C57BL/6J mice were randomly divided into blank,model,positive control,Houttuynia cordata juice low-dose,medium-dose and high-dose groups.All groups except the blank one were induced for UC by using 3%DSS solution.Four days later,the animals were intragastrically administered with physical saline,mesalazine or Houttuynia cordata juice(HCJ)for 10 consecutive days.Disease activity index(DAI)was calculated and the levels of malondialdehyde(MDA),tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),interleukin(IL)-1β,IL-4,IL-6,IL-10,myeloperoxidase(MPO)activity and superoxide dismutase(SOD)activity in the colon tissues of mice,and serum lipopolysaccharides(LPS)and D-lactic acid(D-La)levels were measured.Hematoxylin-eosin(HE)staining was used to evaluate histopathological changes in the colon.The results showed that compared with the model group,hematochezia in the low-,medium-and high-dose HCJ groups was improved,and the mental status was better.The body mass,colon length and thymus index of mice in the medium-and high-dose HCJ groups were significantly increased(P<0.01),DAI,spleen index and the levels of TNF-α,IL-1β,IL-6 and IFN-γwere significantly decreased(P<0.01),the levels of IL-4 and IL-10 were significantly increased(P<0.01),the content of MDA and the activity of MPO were significantly decreased(P<0.01),the activity of SOD was significantly increased(P<0.01),serum D-La and LPS levels were significantly decreased(P<0.01),colon tissue injury was significantly reduced,and pathological score was significantly decreased(P<0.01).In conclusion,Houttuynia cordata juice can improve the inflammatory response of mice with DSS-induced UC,reduce the intestinal permeability,regulate the level of oxidative stress and alleviate intestinal injury.
作者
邓代霞
李凤兰
李潮云
付平
吴天祥
DENG Daixia;LI Fenglan;LI Chaoyun;FU Ping;WU Tianxiang(School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China;Guizhou Vocational College of Foodstuff Engineering,Guiyang 551400,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2023年第1期107-114,共8页
Food Science
基金
国家自然科学基金地区科学基金项目(31460537)。
关键词
鱼腥草
葡聚糖硫酸钠
溃疡性结肠炎
炎症因子
Houttuynia cordata
dextran sodium sulfate
ulcerative colitis
inflammatory factor
