摘要
目的:研究慢性乙型肝炎(CHB)患者外周血性别决定区相关高迁移率族盒蛋白4(SOX4)基因表达水平与临床特征及预后之间的相关性。方法:选取2017年9月-2018年10月于本院收治的97例慢性乙型肝炎患者作为病例组,另外选同期体检健康者30例为正常组,随访2年,比较两组的SOX4、核转录因子阴阳1(YY1)表达水平,采用Spearman法分析CHB患者外周血SOX4 mRNA与临床特征的相关性,采用Cox风险模型分析SOX4表达与CHB患者临床病理特征的关系,采用Kaplan-Meier法分析CHB患者2年生存率曲线。结果:病例组SOX4阳性表达率为41.24%,高于正常组的16.67%(P<0.05);病例组的SOX4 mRNA、YY1 mRNA水平均高于正常组(P<0.05);不同肝功能的CHB患者SOX4、YY1 mRNA水平比较,差异有统计学意义(P<0.05);CHB患者YY1、Child-Pauh分级均与SOX4水平呈正相关(r=0.562,0.438,P<0.05);不同病理分期、病理分级、肝硬化和YY1表达水平的CHB患者的SOX4阳性率比较,差异有统计学意义(P<0.05);肝硬化(RR=1.610)、SOX4高表达(RR=1.473)是影响CHB患者2年疾病进展的独立因素(P<0.05);SOX4低表达患者的2年生存率高于高表达患者(χ^(2)=4316,P=0.038)。结论:SOX4在CHB患者的表达高于健康人群,且高表达SOX4的CHB患者2年生存率低于低表达患者,单因素、多因素的分析结果均显示SOX4高表达是影响CHB患者预后的危险因素,提示SOX4可能参与CHB的发生、发展过程。
Objective:To study the correlation between the expression level of peripheral blood sex-determining region Y box 4(SOX4)gene and clinical features,prognosis in patients with chronic hepatitis B(CHB).Methods:A total of 97 CHB patients admitted to the hospital from September 2017 to October 2018 were enrolled as patient group,while another 30 healthy controls during the same period were enrolled as normal group.After 2-year of follow-up,the expression level of SOX4 and nuclear transcription fector Yin Yang 1(YY1)were compared between the two groups.The correlation between peripheral blood SOX4 mRNA and clinical features in CHB patients was analyzed by Spearman The relationship between SOX4 expression and clinicopathological characteristics in CHB patients was analyzed by Cox hazard model.The curves of 2-year survival rates in CHB patients were analyzed by Kaplan-Meier.Results:The positive expression rate of SOX4 in patient group was higher than that in normal group(41.24%vs 16.67%)(P<0.05),and levels of SOX4 mRNA and YY1 mRNA were higher than those in normal group(P<0.05).The differences in levels of SOX4 and YY1 mRNA among CHB patients with difierent liver function were statistically significant(P<0.05).YY 1 and Child-Pauh grading were positively correlated with SOX4 level(r=0.562,0.438,P<0.05).The differences in positive rate of SOX4 among CHB patients with difierent pathological staging,pathological grading,cirrhosis and YY1 level were statistically significant(P<0.05).Cirrhosis(RR=1.610)and high-expression SOX4(RR=1.473)were independent factors afiecting 2-year disease progression in CHB patients(P<0.05).The 2-year survival rate in patients with low-expression SOX4 was higher than that with high-expression SOX4(χ^(2)=4.316,P=0.038).Conclusion:The expression of SOX4 in CHB patients is higher than that in healthy people.The 2-year survival rate in CHB patients with high-expression SOX4 is lower than that with low-expression SOX4.The results of univariate and multivariate analysis showed that SOX4 high expression is a risk factor affecting prognosis of CHB patients,which indicates that SOX4 may participate in the occurrence and development of CHB.
作者
刘岚宁
LIU Lanning(Department of Laboratory Medicine,Zhengzhou First People's Hospital,Henan Zhengzhou 450000)
出处
《医学检验与临床》
2022年第12期27-31,共5页
Medical Laboratory Science and Clinics
