摘要
为探究不同温度对天麻生长影响的生理分子机理,本研究通过转录组和代谢组联合分析不同温度对天麻基因表达和代谢产物含量的影响,阐明其对天麻生长发育的调控。本研究以13℃母麻(NS)、23℃母麻(TB)和23℃箭麻(SS)为实验材料。转录组分析获得126787个Unigene,注释到6大数据库的Unigene数有59501个,占总Unigene数的46.93%;基于显著差异表达的阈值|log2(变化倍数)/≥1(P<0.05),在NSvsSS、NSvsTB和SSvsTB中分别有17201、17162和10322个差异表达基因。KEGG通路富集分析表明差异表达基因主要参与代谢途径、次生代谢产物的生物合成、RNA转运等途径。代谢组共检测到437个代谢物,其中NSvsSS、NSvsTB和SSvsTB分别获得200、204和87个含量差异代谢物,其中分别有52、51和34个代谢产物含量上调,148、153和53个代谢物含量下调。这些差异物主要分布于糖醇类、氨基酸、有机酸、脂质类、核苷酸、酚酸类和维生素7类物质中。本研究结果表明不同温度通过影响天麻代谢物相关基因表达和代谢物差异变化来调控天麻生长发育,为今后指导天麻栽培、克隆和开发天麻重要性状功能基因提供理论参考。
In order to explore the physiological and molecular mechanisms of the effects of different temperatures on the growth of Gastrodia elata, this study combined the transcriptome and metabolome to analyze the effects of different temperatures on the genes expression and metabolites content of Gastrodia elata and clarify its regulation on the growth and development of Gastrodia elata. In this study, 13 ℃ female hemp(NS), 23 ℃ female hemp(TB)and 23 ℃ arrow hemp(SS) were used as experimental materials. The transcriptome analysis obtained 126 787Unigenes, and 59 501 Unigenes was annotated to 6 major databases, accounting for 46.93% of the total number of Unigenes;Based on the threshold of significant differential expression |log2(Multiple change)|≥1 and P<0.05. There were 17 201, 17 162 and 10 322 DEGs in NS vs SS, NS vs TB and SS vs TB, respectively. Enrichment analysis of KEGG pathway indicated that DEGs were mainly involved in metabolic, biosynthesis of secondary metabolites,and RNA transport pathways. A total of 437 metabolites were detected in the metabolome, of which NS vs SS, NS vs TB and SS vs TB obtained 200, 204 and 87 metabolites with different contents respectively. Among them, the different contents of 52, 51 and 34 metabolites were up-regulated, and 148, 153 and 53 metabolites was down-regulated respectively. These differences were mainly distributed in sugar alcohols, amino acids, organic acids, lipids,nucleotides, phenolic acids and vitamins. The results of this study show that different temperatures regulate the growth and development of Gastrodia elata by affecting the expression of metabolite-related genes and different metabolitaes. This lays the foundation for guiding the cultivation of Gastrodia elata, cloning and developing important functional genes of Gastrodia elata in the future.
作者
周春艳
狄永国
仇全雷
蔡金龙
刘云霞
徐慧妮
李昆志
Zhou Chunyan;Di Yongguo;Qiu Quanei;Cai Jinlong;Liu Yunxia;Xu Huini;Li Kunzhi(College of Life Science and Technology,Kunming University of Science and Technology,Kunming,650500;Agricultural and Rural Bureau of Zhaoyang District,Zhaotong City,Zhaotong,657000;Tibet Bomi Plateau Tibetan Gastrodia Elata Industry Development Co.,LTD.,Ningchi,860300)
出处
《分子植物育种》
CAS
北大核心
2023年第1期110-122,共13页
Molecular Plant Breeding
基金
国家自然科学基金项目(31760349,31960071)
波密县野生藏天麻种质资源收集、保护与利用项目(XZ2021-01YD0015C)共同资助
关键词
天麻
转录组
差异表达基因
代谢组
差异代谢物
Gastrodia elata Blume
Transcriptome
Differentially expressed genes
Metabolome
Differential metabolites
