摘要
目的探究妊娠期高血压疾病(HDCP)患者血清分泌型卷曲相关蛋白5(SFRP5)、miR-124-3p表达水平及临床意义。方法选取金华市妇幼保健院2019年1月至2022年2月确诊的98例HDCP患者为观察组,其中包括妊娠期高血压(GH)组41例,轻度子痫前期(PE)32例,重度PE 25例,另选取同期的80名健康体检者作为对照组。采用酶联免疫吸附法(ELISA)检测SFRP5水平,实时荧光定量法(qRT-PCR)检测miR-124-3p表达水平。分析SFRP5、miR-124-3p水平与HDCP患者临床病理指标的关系,采用Pearson法分析SFRP5与miR-124-3p的相关性。HDCP的危险因素探究利用多因素Logistic回归。结果与对照组相比,观察组血清SFRP5表达水平[(33.78±5.21)vs(43.34±8.56)ng/L]下调,而miR-124-3p水平[(2.16±0.41)vs(1.01±0.17)]上调,且观察组血清SFRP5水平随着疾病程度加重而降低[(38.43±6.37)vs(33.18±5.14)vs(26.94±3.38)ng/L],而miR-124-3p水平随疾病程度加重而升高[(1.62±0.24)(2.19±0.43)(3.01±0.69)],其差异均具有统计学意义(P<0.05)。HDCP患者血清中SFRP5、miR-124-3p表达水平与患者年龄、孕次、孕前BMI、空腹血糖水平有关(P<0.05),而与患者孕周无关(P>0.05)。生物信息学TargetScan网站显示,SFRP5和miR-124-3p存在结合位点。Pearson相关性分析结果显示,SFRP5和miR-124-3p呈负相关(r=-0.610,P<0.05)。回归分析显示,SFRP5是妊娠期孕妇发生HDCP的保护因素,miR-124-3p是危险因素(P<0.05)。结论HDCP患者血清SFRP5、miR-124-3p水平呈异常表达,且和疾病的程度存在一定的关系,二者均调控HDCP的发生和发展。
Objective To explore the expression levels and the clinical significance of serum secreted frizzled-related protein 5(SFRP5)and miR-124-3p in patients with hypertension during pregnancy.Methods Ninety-eight patients with hypertension during pregnancy diagnosed from Jan.2019 to Feb.2022 were selected as the observation group.According to the degree of the condition of patients,they were divided into 41 cases of pregnancy hypertension,32 cases of mild preeclampsia,and 25 cases of severe preeclampsia,and 80 healthy subjects during the same period were selected as the control group.Enzyme-linked immunosorbent assay(ELISA)was used to detect the expression level of serum SFRP5 in patients,real-time fluorescence quantitative method(qRT-PCR)was used to detect the expression level of miR-124-3p.The relationship between SFRP5,miR-124-3p levels and clinicopathological indicators in patients with hypertension in pregnancy was analyzed,Pearson correlation analysis was used to analyze the correlation between SFRP5 and miR-124-3p.Multivariate Logistic regression was used to analyze the risk factors of hypertension in pregnancy.Results Compared with the control group,the serum SFRP5 expression level of the observation group[(33.78±5.21)ng/L vs(43.34±8.56)ng/L]was down-regulated,while the miR-124-3p level[(2.16±0.41)vs(1.01±0.17)]was up-regulated,and the serum SFRP5 level of the observation group decreased with the severity of the disease[(38.43±6.37)ng/L(33.18±5.14)ng/L(26.94±3.38)ng/L],while the level of miR-124-3p increased with the severity of the disease[(1.62±0.24)(2.19±0.43)(3.01±0.69)],the difference was statistically significant(P<0.05).The expression levels of SFRP5 and miR-124-3p in the serum of patients with hypertension in pregnancy were related to the age,pregnancy,pre-pregnancy BMI,and fasting blood glucose level of patients(P<0.05),but not related to the gestational age of patients(P>0.05).Bioinformatics TargetScan website showed that SFRP5 and miR-124-3p had binding sites.Pearson correlation analysis showed that SFRP5 and miR-124-3p were negatively correlated(r=-0.610,P<0.05).Multivariate Logistic regression analysis showed that SFRP5 was a protective factor for pregnancy-induced hypertension in pregnant women,and miR-124-3p was a risk factor(P<0.05).Conclusion The serum levels of SFRP5 and miR-124-3p are abnormally expressed in patients with hypertension during pregnancy,and there is a certain relationship with the degree of disease.Both are involved in the occurrence and development of hypertension during pregnancy.
作者
聂桂兰
范徐妃
王春茶
于辉兰
范俊霞
Nie Guilan;Fan Xufei;Wang Chuncha;Yu Huilan;Fan Junxia(Department of Obstetrics,Jinhua Maternal and Child Health Hospital,Jinhua 321000,China;Department of Obstetrics,Jinhua Central Hospital,Jinhua 321000,China)
出处
《中华内分泌外科杂志》
CAS
2022年第6期693-697,共5页
Chinese Journal of Endocrine Surgery
基金
浙江省基础公益研究计划项目(LQ19H040002)。
