摘要
The three-dimensional(3D)spheroid culture has been widely used as an important tool in biological research.Although several techniques have been established to prepare cell spheroids,fast and controllable production remains one of the major challenges.In this study,a simple but efficient method utilizing the inertial focusing effect in rotating hanging droplets is demonstrated for the rapid and controllable production of cell spheroids.
基金
the Fundamental Research Funds for the Central Universities(Nos.JKF-YG20-B008 and YWF-19-BJ-J-132)
the National Natural Science Foundation of China(Nos.11674019 and 12072010)for the financial support
partially supported by Yunnan Baiyao Group Co.Ltd.