摘要
目的:为探讨普洱茶-纳米硒制备掺杂型碳量子点的可行性及其相关特性,实现水体系中Fe^(3+)的快速检测。方法:以普洱茶水提取物稳定分散的普洱茶-硒原子为掺杂原子,采用水浴法,通过优化反应温度和时间,同时制备出普洱茶-硒掺杂碳量子点(Pu-erh tea nano-selenium doped carbon quantum dots,PT-Se-CQDs)和单质硒两种物质;采用紫外-可见吸收光谱和荧光光谱等技术表征PT-Se-CQDs的紫外-可见吸收特性和荧光强度,采用透射电子显微镜、X射线光电子能谱及X射线衍射等技术表征其形态形貌、元素组成及结构特性;并以PT-Se-CQDs为荧光探针构建荧光传感器,用于水体系中Fe^(3+)检测。结果:当反应温度100℃、反应时间10 h时,可同时制备得量子产率为3.41%、平均直径约为3.1 nm的类球形PT-Se-CQDs和单质硒。Fe^(3+)对PT-Se-CQDs具有强荧光静态猝灭效应,当Fe^(3+)浓度为0~300μmol/L时,比率荧光强度(F/F_(0))与Fe^(3+)浓度呈良好的线性关系(R^(2)>0.99),Fe^(3+)的检出限低至0.2621μmol/L;纯净水和矿泉水中Fe^(3+)含量测定的加标回收率分别为90.93%~104.56%和84.53%~113.90%,RSD小于8.15%和4.00%。结论:本研究制备的PT-Se-CQDs对Fe^(3+)具有高选择性和灵敏度,以此建立的检测Fe^(3+)方法简单、快速,具有良好的应用前景。
Object:To prepare Pu-erh tea nano-selenium doped carbon quantum dots(PT-Se-CQDs)for the rapid detection of Fe^(3+)in the water system and to profile their characteristics.Method:In this study,PT-Se-CQDs and elemental selenium were prepared simultaneously in a water-bath by optimizing the reaction temperature and time.The ultraviolet-visible absorption and fluorescent intensity of PT-Se-CQDs were subsequently analyzed by the ultraviolet-visible absorption spectroscopy and fluorescence spectroscopy.And their morphology,elemental composition,and structural characteristics were characterized by transmission electron microscopy,X-ray photoelectron spectroscopy,and X-ray diffraction,respectively.On this basis,a novel fluorescence sensor for the detection of Fe^(3+)in the aqueous system was constructed using PT-Se-CQDs.Result:PT-Se-CQDs in a spherical shape with a quantum yield of 3.41%,an average particle size of about 3.1 nm as well as elemental selenium were successfully prepared simultaneously via the reaction in a boiling water bath at 100℃for 10 h.In addition,a strong static fluorescence quenching effect on PT-Se-CQDs was observed in the presence of Fe^(3+).Accordingly,Fe^(3+)in the range of 0~300μmol/L was successfully detected using PT-Se-CQDs as a fluorescence sensor with a good linear relationship between the concentration of Fe^(3+)and the ratio of fluorescence intensity(F/F_(0))of PT-Se-CQDs(R~2>0.99)and a limit of detection of 0.2621μmol/L.When this method was applied to detect Fe^(3+)in real water samples,satisfactory standard recovery rates of Fe^(3+)in pure water and mineral water of 90.93%~104.56%and 84.53%~113.90%with the RSD less than 8.15%and 4.00%were obtained,respectively.Conclusion:The preparation of PT-Se-CQDs with high selectivity and sensitivity to Fe^(3+)and their application as a new fluorescence sensor for the detection of Fe^(3+)in aqueous systems with simple operation and fast response were explored in the present study.
作者
胡伟英
叶锡光
陈忠正
张媛媛
姚向荣
林晓蓉
李斌
HU Weiying;YE Xiguang;CHEN Zhongzheng;ZHANG Yuanyuan;YAO Xiangrong;LIN Xiaorong;LI Bin(College of Food Science,South China Agricultural University,Guangzhou 510642,China)
出处
《食品工业科技》
CAS
北大核心
2023年第3期316-324,共9页
Science and Technology of Food Industry
基金
国家自然科学基金青年科学基金项目(31901652)
现代农业产业技术体系专项资金(CARS-19)
广州市科技计划项目(202102020236)。
关键词
普洱茶-纳米硒
普洱茶-硒掺杂碳量子点
单质硒
荧光传感器
FE3+
Pu-erh tea nano-selenium
Pu-erh tea nano-selenium doped carbon quantum dots(PT-Se-CQDs)
elemental selenium
fluorescence sensor
Fe3+
