摘要
目的利用核酸适配体W3对乳腺癌细胞的结合特异性,对乳腺癌细胞以及临床乳腺癌组织石蜡切片标本进行量子点(QDs)成像。方法利用流式细胞测量术检测核酸适配体W3对乳腺癌细胞系的结合特异性,基于生物素与链霉亲和素的结合反应原理,通过荧光显微镜观察核酸适配体W3对乳腺癌细胞系以及临床乳腺癌患者组织标本的量子点靶向成像,并进一步分析其临床意义。结果核酸适配体W3能特异性结合恶性程度较高的乳腺癌细胞,偶联量子点QD605形成量子点探针(W3-QDs),能够对恶性程度较高的乳腺癌细胞系以及乳腺癌组织实现靶向成像,并且具有高恶性程度的乳腺癌组织的荧光强度明显高于恶性程度低的乳腺癌组织。结论核酸适配体W3偶联量子点后能够实现乳腺癌的靶向成像,可用于乳腺癌的早期诊断。
Objective To perform quantum dots(QDs)imaging of breast cancer cells and breast cancer tissue paraffin specimens by using nucleic acid aptamer W3 binding specificity on breast cancer cells.Methods The binding specificity of nucleic acid aptamer W3 to breast cancer cell lines was detected by flow cytometry,and based on the binding reaction principle of biotin and streptomyces avidin,the quantum dot targeting imaging of nucleic acid aptamer W3 on breast cancer cell lines and tissue specimens of clinical patients was observed by fluorescence microscopy,and its clinical significance was further analyzed.Results The nucleic acid aptamer W3 specifically binded to breast cancer cell lines with high malignancy,and after coupling quantum dots QD605(W3-QDs),it achieved targeted imaging of breast cancer cell lines and breast cancer tissues with high malignancy,and the fluorescence intensity of breast cancer tissues with high malignancy degree was significantly higher than that of breast cancer tissues with low malignancy.Conclusions The nucleic acid aptamer W3 may achieve target imaging of breast cancer after coupling quantum dots,which can be used for early diagnosis of breast cancer.
作者
王群
李鑫
马俊
李婉明
WANG Qun;LI Xin;MA Jun;LI Wanming(Department of Molecular Cell Biology,Key Laboratory of Medical Cell Biology,Ministry of Education,Key Laboratory of Cell Biology,Ministry of Public Health,China Medical University,Shenyang 110122,China)
出处
《基础医学与临床》
2023年第1期76-82,共7页
Basic and Clinical Medicine
基金
辽宁省自然科学基金(2020-MS-164)
沈阳市中青年科技创新人才支持计划(RC190261)。
关键词
适配体
乳腺癌
量子点
靶向成像
aptamer
breast cancer
quantum dot
targeted imaging
