摘要
为了探究浙江大学教学动物医院就诊患猫鼻窦炎眶内感染的病原菌特点,试验采集患猫额窦、鼻腔、鼓泡部位的内容物,采用细菌分离纯化、16S rDNA基因序列分析对分离菌株进行鉴定;通过药敏试验、小鼠致病性试验、全基因组序列分析对分离菌株进行耐药性、致病性分析和遗传进化分析。结果表明:仅从患猫鼓泡内容物中分离得到1株铜绿假单胞菌,命名为ZJU2021。ZJU2021属于多重耐药菌株,对左氧氟沙星、氨曲南、多黏菌素B等5种药物敏感,而对美罗培南、万古霉素、多西环素等30种药物耐药。ZJU2021菌株基因组全长为6868280 bp,含有6426个基因;其中氨基糖苷类、氟喹诺酮类、β-内酰胺类等药物相关耐药基因48个;抗生素抗性基因61个,形成抗生素外排、抗生素靶向、抗生素灭活等耐药机制;毒力因子编码基因687个,其产物主要关于抗菌活性、铁摄取、调控系统、毒性蛋白等。ZJU2021毒力基因toxA和exoT与GenBank中的铜绿假单胞菌相应基因核苷酸相似性高于99%,其中toxA与从天津包装饮用水中分离的铜绿假单胞菌TJ2019-022株(登录号为CP065865.1)位于同一分支上,且二者构成独立分支;exoT基因与北京、兰州等地分离的人源铜绿假单胞菌处于同一分支。说明本次分离得到的菌株具有较强的致病性,其耐药基因在人与动物之间的传播会造成严重的公共卫生安全问题,因此临床上应加强人兽共患细菌性疾病的监测。
In order to explore the characteristics of pathogenic bacteria within the in-frame of feline sinusitis in the Teaching Animal Hospital of Zhejiang University,in this experiment the contents of frontal sinus,nasal cavity and bullae of sick cats were collected;the isolated bacteria were identified by bacterial isolation and purification,and 16 S rDNA gene sequence analysis;the drug resistance,pathogenicity and genetic evolution of the isolated strains were analyzed by drug susceptibility test,mouse pathogenicity test and whole genome sequence analysis.The results showed that only one strain of Pseudomonas aeruginosa was isolated from the bullae contents of the cat,named ZJU2021.ZJU2021 belonged to a multi-drug resistant strain,and was sensitive to 5 drugs such as levofloxacin,aztreonam and polymyxin B;it was resistant to 30 drugs such as meropenem,vancomycin and doxycycline.The full-length genome of strain ZJU2021 was 6868280 bp,encoding 6426 genes,containing 48 drug-related resistance genes for aminoglycosides,fluoroquinolones,andβ-lactams;61 antibiotic resistance genes producing resistance mechanisms such as antibiotic efflux,antibiotic targeting,and antibiotic inactivation,and 687 genes encoding virulence factors whose products were mainly related to antibacterial activity,iron uptake,regulatory systems,and toxic proteins.The nucleotide similarity of ZJU2021 virulence genes toxA and exoT with the corresponding genes of Pseudomonas aeruginosa in GenBank was higher than 99%.Among them,its toxA and Pseudomonas aeruginosa TJ2019-022 strain(accession number CP065865.1)isolated from Tianjin packaged drinking water were located on the same branch,and the two strains constituted to an independent branch.Its exoT gene was in the same branch as the human-derived Pseudomonas aeruginosa isolated from Beijing,Lanzhou and other place.The results suggested that the strain isolated in this experiment had strong pathogenicity,and the transmission of drug resistance genes between humans and animals would cause serious public health and safety problems.Therefore,clinical monitoring of zoonotic bacterial diseases should be strengthened.
作者
李慧敏
李培德
潘嘉臻
马晓丹
任大喜
王华南
冷超粮
LI Huimin;LI Peide;PAN Jiazhen;MA Xiaodan;REN Daxi;WANG Hua'nan;LENG Chaoliang(Henan Provincial Engineering and Technology Center for Animal Disease Diagnosis and Comprehensive Prevention and Control/Henan Provincial Engineering Laboratory of Insects Bioreactor,Nanyang Normal University,Nanyang 473061,China;College of Animal Science,Wenzhou Vocational College of Science and Technology,Wenzhou 325006,China;Wenzhou City Pet Engineering Research Center,Wenzhou 325006,China;College of Animal Sciences,Zhejiang University,Hangzhou 310000,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2022年第24期62-68,75,136,137,共10页
Heilongjiang Animal Science And veterinary Medicine
基金
浙江省重点研发计划项目(2021C02060)
温州市宠物工程技术研究中心开放课题(WP03)。
关键词
铜绿假单胞菌
分离
鉴定
药敏试验
全基因组分析
毒力基因
Pseudomonas aeruginosa
isolation
identification
drug sensitivity test
whole genome analysis
virulence gene
