摘要
为研究我国北方地区鼻咽癌和非鼻咽癌患者Epstein Barr病毒(EBV)潜伏膜蛋白1(LMP1)基因C端区缺失状况,探讨其在鼻咽癌发生中所起的作用。收集我国东北地区鼻咽癌石蜡组织22例,非鼻咽癌患者外周血26例,提取DNA后,采用聚合酶链反应技术(PCR)扩增LMP1基因的C末端,对其中有缺失的4例鼻咽癌进行了克隆和序列分析。结果显示:22例鼻咽癌组织标本中,有19例扩增出特异性条带,阳性率为86%。其中8例存在缺失,缺失率42%。取4例缺失样品进行序列分析,并与B95-8原型LMP1做比较,结果显示:4例鼻咽癌样品均存在30个碱基的缺失和某些位点的单点突变,并由此引起所编码的氨基酸改变。26例非鼻咽癌患者LMP1阳性扩增率为92 31%,无一例缺失。此结果表明:与广东、广西鼻咽癌高发区相比,我国北方地区鼻咽癌与非鼻咽癌患者中EB病毒LMP1基因缺失型和原型的分布有明显的地区差异。
Sera from 113 NPC patients, 100 normal individuals and 40 patients with tumor other than NPC were tested for EBV IgA/MA antibody by indirect immunofluorescence ( IF ) test. The transformed C-127 mous cells ( PO4 cells ) producing EBV membrance antigen gp340/220 were used as target cells for the detection of IgA/MA antibqdy.The positive rate of IgA/MA antibody in NPC patients, normal individuals and patients with tumor other than NPC was 91.15%, 1% and 0% respectively. There is marked difference between the NPC patients group and other groups. These data further indicated that the IgA/MA antibody is very specific for NPC. The procedure for detection of IgA/MA antibody by using P04 cells fixed on slides instead of B95-8 cells was simple and convenient.
出处
《病毒学报》
CAS
CSCD
北大核心
2002年第4期307-311,共5页
Chinese Journal of Virology
基金
国家自然科学基金重点项目(39830380)
