摘要
该研究以13种葡萄砧木品种为材料,采用盆栽试验用100 mmol/L NaCl的1/2 Hoagland s营养液浇灌处理,以葡萄砧木无盐胁迫为对照,待砧木长至20片完全展开叶后测定各品种叶片的相对电导率、渗透调节物质含量、MDA含量、活性氧含量、抗氧化酶活性等指标,探究不同葡萄砧木耐盐能力及其机制。结果显示:(1)至盐胁迫处理结束时,葡萄砧木‘188-08’、‘3309C’、‘140R’的处理组叶片近乎完全脱落,‘贝达’、‘5BB’、‘Dogridge’有少部分叶片脱落,而‘101-14’和‘110R’叶枯现象最少。(2)盐胁迫条件下,耐盐性较差的品种‘3309C’和‘140R’叶片相对电导率均大于90%,而耐盐性较强的品种‘101-14’和‘110R’的叶片相对电导率在60%左右;随着盐胁迫处理天数的增加,‘110R’的MDA含量呈明显的上升趋势。(3)盐胁迫条件下,耐盐性较强的品种‘101-14’和‘110R’积累了较多的可溶性蛋白、可溶性糖、脯氨酸等渗透调节物质,而耐盐性弱的品种‘3309C’则积累了较多的活性氧,引起明显的脂膜过氧化,严重影响砧木的正常代谢。(4)在盐胁迫条件下,耐盐品种‘101-14’的SOD、POD等抗氧化酶活性显著提高,有效清除体内过量的活性氧,从而维持细胞膜的稳定性。研究发现,葡萄砧木品种‘101-14’、‘110R’在盐胁迫下能积极积累较多渗透调节物质,增强自身抗氧化酶活性,表现出较强的渗透调节和抗氧化能力,有效抵御盐胁迫造成的伤害,可作为西北盐渍化地区葡萄栽培的砧木品种。
In order to study the capability and mechanism of different grape rootstocks under salt stress,we used 13 grape rootstocks as experimental materials.The grapevines were supplied with 1/2 Hoagland s nutrient solution with 100 mmol/L NaCl,grapevine rootstocks which were not treated with 100 mmol/L NaCl were used as control.After 20 fully expanded true leaf,we investigated the relative conductivity,osmotically regulates substance content,MDA content,antioxidant enzyme activities of leaves and roots.The results showed:(1)the leaves of the‘188-08’,‘3309C’and‘140R’treatment groups were almost completely shed,and a small number of leaves fell off in the‘Beta’,‘5BB’and‘Dogridge’,and the leaf wilting phenomena of‘101-14’and‘110R’were the least until grape rootstock leaves to the end of the salt stress treatment.(2)Under salt stress,the relative conductivity of leaves of the varieties with poor salt tolerance such as‘3309C’and‘140R’was greater than 90%,and the relative conductivity of leaves of the varieties with strong salt tolerance such as‘101-14’and‘110R’was about 60%.At the same time,with the increase in salt stress treatment days,the MDA content of‘110R’showed a clear upward trend.(3)Under salt stress,the higher salt-tolerant varieties of‘101-14’and‘110R’produced more of osmoregulatory substances such as soluble proteins,soluble sugars and proline.The‘3309C’cultivar with poor salt tolerance has accumulated more reactive oxygen species,causing significant lipid film peroxidation,which seriously affects the normal metabolism of rootstock.(4)Under salt stress,‘101-14’improved the activities of antioxidant enzymes such as SOD and POD,removed excessive reactive oxygen in the body and maintaining the stability of the cell membrane.The results show that grape rootstock varieties‘101-14’and‘110R’can actively accumulate more osmotic regulatory substances under salt stress,enhance their antioxidant enzyme activities,show strong osmotic regulation and antioxidant capacity,and effectively resist the damage caused by salt stress.Therefore,it can be used as a rootstock variety in viticulture in the salinized areas of the northwest region.
作者
陈泽平
史晓敏
王瑞
吴轩
王宁
王振平
CHEN Zeping;SHI Xiaomin;WANG Rui;WU Xuan;WANG Ning;WANG Zhenping(School of Agricultural,Ningxia University,Yinchuan 750021,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2022年第11期1880-1891,共12页
Acta Botanica Boreali-Occidentalia Sinica
基金
财政部和农业农村部国家现代农业产业技术体系(CARS-29-zp-3)
宁夏重大科技攻关项目(2018BBF02015)。
关键词
葡萄
砧木
盐胁迫
叶片
活性氧代谢
渗透调节
grapevine
stock
salt stress
blade
active oxygen metabolism
osmotic regulation
