摘要
从健康多花黄精根部分离筛选对黄精种子萌发具促生作用的内生细菌,以期为解决黄精资源短缺问题奠定理论基础。采用传统组织培养法分离纯化内生菌,沙尔科夫斯基反应(Salkowski法)筛选产IAA能力的促生菌;通过形态学观察、生理生化特征检测及16S rDNA序列分析确定目标菌株的分类地位;温室纸床实验验证目标菌株对黄精种子发芽的促生效果。从多花黄精中共分离纯化得到34株内生菌,其中细菌25株,真菌9株;从中筛选出两株具有稳定高产IAA的内生细菌HNC2和HPB1,其产IAA能力分别高达4.833 mg/L、4.608 mg/L;菌种鉴定结果表明菌株HNC2和HPB1分别为芽孢杆菌(Bacillus sp.)和葡萄球菌(Staphylococcus sp.);菌种对种子萌发影响的实验结果显示,与无菌水和培养基对照组比,促生菌Bacillus sp.HNC2和Staphylococcus sp.HPB1均显著提高黄精种子的发芽势与发芽率,且对萌发幼苗的生长具有不同程度的促生功效。多花黄精具有丰富的内生菌资源,Bacillus sp.HNC2和Staphylococcus sp.HPB1有望进一步开发为微生物菌肥以提高黄精种子的繁殖率。
The endophytic bacteria that can promote the seed germination of Polygonatum were isolated and screened from the healthy roots of Polygonatum cyrtonema Hua.,in order to lay a theoretical foundation for solving the shortage of Polygonatum resources.The endophytes were isolated and purified by the traditional tissue culture method,and the IAA-producing bacteria were screened by the Salkowski reaction(Salkowski method).The taxonomic status of the target strains was determined by morphological observation,physiological and biochemical characterization,and 16S rDNA sequence analysis.The germination-promoting effect of the target strains on the seeds of Polygonatum was verified by greenhouse paper-bed experiments.The results showed that 34 endophytes were isolated and purified from Polygonatum cyrtonema Hua.,including 25 endophytic bacteria and 9 endophytic fungi.Two endophytic bacteria HNC2 and HPB1 with stable and high content of IAA were screened out,and their IAA production capacity was as high as 4.833 mg/L and 4.608 mg/L,respectively.The results of bacterial identification showed that strains HNC2 and HPB1 were Bacillus sp.and Staphylococcus sp.,respectively.The experimental results regarding the effect of the strains on seed germination showed that both of the growth-promoting bacteria Bacillus sp.HNC2 and Staphylococcus sp.HPB1significantly increased the germination potential and germination rate of Polygonatum seeds compared to the sterile water and medium controls,and had different degrees of growth-promoting effects on the growth of seedlings.Polygonatum cyrtonema Hua.harbors abundant endophytic bacteria,of which Bacillus sp.HNC2 and Staphylococcus sp.HPB1 are expected to be further developed as microbial fertilizer to increase the germination rate of Polygonatum seeds.
作者
杜佳慧
徐伟芳
杨晓冬
谭松
尹登科
刘园旭
DU Jia-hui;XU Wei-fang;YANG Xiao-dong;TAN Song;YIN Deng-ke;LIU Yuan-xu(School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012;Institute of Pharmaceutics in Anhui Academy of Chinese Medicine,Hefei 230012;Anhui Province Key Laboratory of Research&Development of Chinese Medicine,Hefei 230012)
出处
《生物技术通报》
CAS
CSCD
北大核心
2022年第12期223-232,共10页
Biotechnology Bulletin
基金
安徽省自然科学基金项目(2108085QH374)
中药研究与开发安徽省重点实验室开放基金(AKLPDCM202008)
安徽中医药大学人才支持项目(2020rcyb008)。
关键词
多花黄精
内生菌
筛选
吲哚乙酸
促生作用
Polygonatum cyrtonema Hua.
endophytes
screening
indole acetic acid
plant-growth-promoting effect
