摘要
目的研究蛇菰多糖(balanophora polysaccharide,BPS)对大鼠乙酸所致胃溃疡(gastric ulcer,GU)的治疗效果,并探讨其作用机制。方法60只♂SD大鼠随机分为假手术组,GU模型组,奥美拉唑阳性组(3.6 mg·kg^(-1)),BPS低、中、高剂量治疗组(100、200、400 mg·kg^(-1))。用乙酸烧灼法制备GU模型,肉眼结合HE染色观察溃疡组织形态和病理变化,测量并计算各组溃疡面积和抑制率;酶法检测超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量和谷胱甘肽过氧化物酶(GSH-PX)活力;ELISA法检测肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)含量;免疫组化法和Western blot检测表皮生长因子(EGF)和表皮生长因子受体(EGFR)的表达水平。结果与假手术组比较,模型组大鼠可见明显溃疡损伤;与模型组比较,BPS治疗组溃疡面积明显减小,SOD和GSH-PX活力升高,EGF和EGFR表达水平升高,MDA、TNF-α和IL-6含量明显降低。结论BPS对大鼠GU具有治疗作用,其作用机制可能与抑制氧化应激,抑制炎症刺激,促进胃黏膜再生修复有关。
Aim To study the therapeutic effect of Balanophora polysaccharide(BPS)on gastric ulcer(GU)induced by acetic acid in rats and to investigate its mechanisms.Methods Sixty male SD rats were randomly divided into sham-operated group,GU model group,omeprazole positive group(3.6 mg·kg^(-1)),and low,medium and high dose of BPS treatment groups(100,200 and 400 mg·kg^(-1)).The GU model group was prepared by acetic acid cautery method,and the morphology and pathological changes of ulcers were observed by visual observation combined with HE staining,and the ulcer area and inhibition rate were measured and calculated;superoxide dismutase(SOD)activity,malondialdehyde(MDA)content and glutathione peroxidase(GSH-PX)activity were measured by enzymatic assay;tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)content were detected by ELISA.The expression levels of epidermal growth factor(EGF)and epidermal growth factor receptor(EGFR)were measured by immunohistochemistry staining and Western blot.Results Compared with the sham-operated group,obvious ulcer damage was seen in the model group.Compared with the model group,the BPS-treated group showed a significant reduction in ulcer area,an increase in SOD and GSH-PX activity and EGF and EGFR expression levels,and a significant decrease in MDA,TNF-αand IL-6 content.Conclusions BPS has a therapeutic effect on GU in rats,and its mechanism may be related to the inhibition of oxidative stress,suppression of inflammatory stimuli and promotion of regenerative repair of gastric mucosa.
作者
夏德尧
黄晟哲
赵依如
王江华
牟俊彦
赵方毓
王凤杰
陈显兵
XIA De-yao;HUANG Sheng-zhe;ZHAO Yi-ru;WANG Jiang-hua;MOU Jun-yan;ZHAO Fang-yu;WANG Feng-jie;CHEN Xian-bing(Dept of pathology,Affiliated Minda hospital,Enshi Hubei 445000,China;Dept of Medical School,Hubei Minzu University,Enshi Hubei 445000,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2023年第1期193-199,共7页
Chinese Pharmacological Bulletin
基金
国家自然科学基金项目(No81660672)
湖北省卫生健康委中医药科研面上项目(NoZY2021M033)
湖北省大学生创新创业训练计划项目(NoS202010517050)。
关键词
蛇菰多糖
胃溃疡
氧化应激
炎症
表皮生长因子
表皮生长因子受体
Balanophora polysaccharide
gastric ulcer
oxidative stress
inflammation
epidermal growth factor
epidermal growth factor receptor
