摘要
透射电子显微镜成像技术广泛应用于结构生物学研究、生物制药、医学病理、纳米材料等领域。近些年,透射电镜中的冷冻电镜技术有了迅速发展,并在2017年获得诺贝尔化学奖。冷冻电镜的快速发展离不开新型探测相机以及低剂量显微照片处理算法的发展。本文回顾了透射电镜相机系统近些年的发展历史,并针对单颗粒冷冻电镜的数据预处理流程做了详细分解。
Transmission Electron Microscopy(TEM) has been widely used in the field of structural biology,drug discovery,pathology,material science and nanotechnology.In recent years,cryogenic electron microscopy(Cryo-EM),awarded the 2017 Nobel Prize in Chemistry,has been rapidly developed.The “resolution revolution” of Cryo-EM is closely related to the synchronous development of new detectors and algorithms for low dose micrograph.This work reviews the recent development of detectors for imaging systems used in TEM,and presents the detailed description of the data preprocessing for single particle by Cryo-EM.
作者
汪进鸿
陈朕欣
邓竞
欧嘉城
曾安
金亮
WANG Jin-hong;CHEN Zhen-xin;DENG Jing;OU Jia-cheng;ZENG An;JIN Liang(Bioland Laboratory(Former Guangzhou Regenerative Medicine and Health-Guangdong Laboratory),Guangzhou Guangdong 510005;School of Computer Science and Technology,Guangdong University of Technology,Guangzhou Guangdong 510006,China)
出处
《电子显微学报》
CAS
CSCD
北大核心
2022年第6期654-663,共10页
Journal of Chinese Electron Microscopy Society
基金
广东省重点领域研发计划项目(No.2020B0303090003)
国家自然科学基金资助项目(No.32027901)。
关键词
透射电镜
冷冻电镜
电子探测器
图像处理
transmission electron microscopy
Cryo-electron microscopy
electron detectors
image processing
