摘要
【目的】揭示褐飞虱Nilaparvata lugens对呋虫胺抗药性形成的分子机制。【方法】以呋虫胺不同抗性的褐飞虱种群为材料,采用qRT-PCR方法检测CYP6ER1在褐飞虱各发育阶段和不同组织以及在呋虫胺半致死浓度处理后的P450酶的活性和CYP6ER1基因的表达情况。采用生物测定的方式检测了广东省不同地理种群褐飞虱对呋虫胺的抗药性和CYP6ER1基因在不同地理种群中的表达量。【结果】CYP6ER1基因在抗性褐飞虱种群中表达量显著高于敏感种群,在成虫期3-9 d时和中肠组织中也有很高的表达量;与对照组相比,褐飞虱在呋虫胺半致死浓度处理后,CYP6ER1基因表达量和P450酶的活性均存在差异;而且广东地区褐飞虱不同地理种群CYP6ER1基因表达量和抗性水平有关。【结论】CYP6ER1可能参与褐飞虱对呋虫胺的解毒代谢过程,影响褐飞虱对呋虫胺的抗药性。
[Objectives]To investigate the molecular mechanism responsible for the resistance of Nilaparvata lugens to dinotefuran.[Methods]The expression of the CYP6ER1 gene and the activity of P450 enzymes were measured and compared in brown planthopper(BPH)populations with different levels of resistance to dinotefuran.The expression of the CYP6ER1 gene in different developmental stages and tissues and the activity of P450 enzymes,were measured after treatment with semi-lethal concentrations of dinotefuran.Susceptibility to dinotefuran was tested using the stem-dip method in a laboratory.[Results]The CYP6ER1 gene was significantly more highly expressed in resistant than in sensitive,populations.It was also more highly expressed in 3 to 9 day-old adults than in other age groups,and in midgut tissue than in other tissues.BPHs treated with semi-lethal concentrations of dinotefuran had significantly different CYP6ER1 gene expression and P450enzyme activity to the control group.[Conclusion]There is a correlation between expression of the CYP6ER1 gene and resistance in different N.lugens populations.CYP6ER1 may therefore be involved in the detoxification of dinotefuran in the brown planthopper.
作者
肖汉祥
李燕芳
陈珊媛
戴阳朔
袁龙宇
张振飞
XIAO Han-Xiang;LI Yan-Fang;CHEN Shan-Yuan;DAI Yang-Shuo;YUAN Long-Yu;ZHANG Zhen-Fei(Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;Guangdong Provincial Key Laboratory of High Technology for Plant Protection,Guangzhou 510640,China)
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2022年第5期1039-1047,共9页
Chinese Journal of Applied Entomology
基金
国家重点研发计划项目(2021YFD1401100)
广东省粤穗联合基金项目(2020A1515110514)。
关键词
褐飞虱
抗药性
CYP6ER1
基因表达
Nilaparvata lugens
pesticide resistance
CYP6ER1
differential expression
