摘要
单细胞成像可在单细胞水平观测目标物位置、确定目标物含量,在生命科学与临床医学研究领域应用广泛.核酸编码扩增技术利用特定分子反应将待测目标识别转化为核酸条码的扩增,具有探针种类多、易编程、反应条件温和及信号放大效率高等特点,在单细胞低丰度、高灵敏、多目标物成像中优势显著,为理解细胞状态、探索生命过程提供了新思路.本文综合评述了核酸编码扩增在单细胞荧光成像领域的研究进展,以目标物的编码方式为分类依据,系统阐述了固定细胞原位成像和活细胞成像中不同目标物编码与扩增成像方式的区别,并对活细胞成像中多重检测面临的问题以及未来发展前景进行了展望.
Single-cell imaging is of great significance in the field of life science and clinical medicine for its applica⁃tion on visualizing and quantifying of targets in single cells.Nucleic acids-encoded amplification converts targets to nucleic acids barcodes through specific molecular reactions,which is easy to achieve signal amplification.Due to its variety of probes,ease of programming,mild reaction conditions and high amplification efficiency,nucleic acidsencoded amplification emerges outstanding performance on sensitive and multiplexed imaging of various targets with low abundance in single cells,emerging as a new strategy for understanding cell state and exploring life process.This paper reviews the research progress of nucleic acids-encoded amplification in the field of single-cell fluorescence imaging on the basis of the encoding methods,which systematically clarifies the characteristics of encoding and amplification methods for living cell imaging and in situ cell imaging.Finally,the challenges of multiplex detection in living cells and future perspectives of nucleic acids-encoded amplification are summarized and discussed.
作者
赵雪琪
赵越
薛静
白敏
陈锋
孙颖
宋大千
赵永席
ZHAO Xueqi;ZHAO Yue;XUE Jing;BAI Min;CHEN Feng;SUN Ying;SONG Daqian;ZHAO Yongxi(College of Chemistry,Jilin Province Research Center for Engineering and Technology of Spectral Analytical Instruments,Jilin University,Changchun 130012,China;School of Life Science and Technology,Institute of Analytical Chemistry and Instrument for Life Science,Key Laboratory of Biomedical Information Engineering of Ministry of Education,Xi’an Jiaotong University,Xi’an 710049,China)
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2022年第12期232-247,共16页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:22125404,21874105,22004096,92068118)资助。
关键词
单细胞分析
核酸编码
核酸扩增
荧光成像
Single-cell analysis
Nucleic acids encoding
Nucleic acids amplification
Fluorescence imaging
