摘要
苦杏仁在储藏过程中容易出现走油变质等情况,故苦杏仁在临床使用时常要求“临方捣碎”。为进一步确认苦杏仁“临方捣碎”的科学理论价值,确定苦杏仁的最佳储存条件,本研究采用UPLC-MS/MS技术对不同储存状态(捣碎和未捣碎)、储存时间(0、2和4周)和储存温度(25℃和4℃)下的苦杏仁进行化学成分比较研究。经文献比对、课题组前期工作积累及Compound Discoverer软件检索,共从苦杏仁提取液中鉴定出58种物质。同时,发现不同储存状态、储存时间和储存温度的苦杏仁化学成分含量具有明显的统计学差异。研究结果表明,未捣碎储存相较于捣碎储存的苦杏仁化学成分组成稳定;4℃储存的相较于25℃储存的苦杏仁化学组成更稳定;储存时间越短,其化学成分变化越少。苦杏仁的主要药效成分苦杏仁苷在以上三种储存条件下均表现出统计学差异,可作为苦杏仁“临方捣碎”的潜在质量标志物。
Bitter almonds(Semen Armeniacae Amarum)are prone to oil deterioration during storage,so they often require mashing prior to clinical use.To confirm the medical value of bitter almonds"being mashed when used"and to determine the optimal storage conditions for bitter almonds,UPLC-MS/MS was used to perform a comparative study of the chemical composition of bitter almonds in different storage states(mashed and unmashed),storage times(0,2 and 4 weeks),and storage temperatures(25℃ and 4℃).A total of 58 substances were identified in bitter almond extracts through literature review,this group’s previous work,and a Compound Discoverer software search.Statistically significant differences were found in the chemical composition and content of bitter almonds in different storage states,storage times,and storage temperatures.The results show that the chemical composition of bitter almonds stored unmashed was more stable than that of bitter almonds stored mashed;the chemical composition of bitter almonds stored at 4℃ was more stable than that of bitter almonds stored at 25℃;and the shorter the storage time,the less the chemical composition changed.Amygdalin,the main medicinal component of bitter almonds,showed statistically significant differences in content under the above three storage conditions,which can be used as a potential quality marker for bitter almonds.
作者
程遥
毕玥琳
冯欣
王佳琪
徐浩然
张童画
于庚原
张晨宁
王景红
孙毅坤
CHENG Yao;BI Yue-lin;FENG Xin;WANG Jia-qi;XU Hao-ran;ZHANG Tong-hua;YU Geng-yuan;ZHANG Chen-ning;WANG Jing-hong;SUN Yi-kun(Department of Pharmacy,Wangjing Hospital,Chinese Academy of Traditional Chinese Medicine,Bejing 100102,China;College of Traditional Chinese Medicine,Beijing University of Traditional Chinese Medicine,Beijing 102488,China)
出处
《药学学报》
CAS
CSCD
北大核心
2022年第10期3195-3202,共8页
Acta Pharmaceutica Sinica
基金
中国西藏自治区科技攻关项目(XZ201801-GA-16)。
