期刊文献+

一例类NADC30猪繁殖与呼吸综合征病毒感染的诊断及防控 被引量:1

在线阅读 下载PDF
导出
摘要 2021年12月初,河北望都县某猪场出现疑似猪繁殖与呼吸综合征(PRRS)疫情,主要表现为繁殖母猪流产、仔猪高热和呼吸困难等。为确定发病原因,对病死仔猪进行剖检,发现仔猪全身淋巴结肿大、肺脏肿大充血等,采集病猪肺脏、淋巴结和扁桃体等组织样品进行实验室诊断。结果发现,送检的2份样品均为猪繁殖与呼吸综合征病毒(PRRSV)阳性,但其它病原核酸均为阴性。以RT-PCR法对该毒株ORF5基因部分序列进行扩增与分析,发现该毒株与类NADC30毒株对应序列同源性较其它基因型PRRSV毒株同源性更高。以上结果提示,该场暴发PRRS是类NADC30毒株感染所致,可采取疫苗接种和提高生物安全防控水平等方式对该病进行科学防控。
出处 《养猪》 2022年第6期87-89,共3页 Swine Production
  • 相关文献

参考文献6

二级参考文献38

  • 1郭宝清,陈章水,刘文兴,崔益洙.从疑似PRRS流产胎儿分离PRRSV的研究[J].中国畜禽传染病,1996(2):1-5. 被引量:955
  • 2童光志,周艳君,郝晓芳,田志军,仇华吉,彭金美,安同庆.高致病性猪繁殖与呼吸综合征病毒的分离鉴定及其分子流行病学分析[J].中国预防兽医学报,2007,29(5):323-327. 被引量:395
  • 3Larochelle R,Magar R.Evaluation of the presence of porcine reproductive and respiratory syndrome virus in packaged pig meat using virus isolation and polymerase chain reaction (PCR)method[J].Vet Microbiol,1997,58:1-8.
  • 4Chung W B,Chan W H,Chaung H C,et al.Real-time PCR for quantitation of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in naturally-infected and challenged pigs[J].J Virol Met,2005,124:11-19.
  • 5Yoon I J,Joo H S,Christianson W T,et al.An indirect fluorescent antibody test for the detection of antibody toswine infertility and respiratory syndrome virus in swine sera[J].J Vet Diagn Invest,1992,4:144-147.
  • 6Qiu H J,Tian Z J,Tong G Z,et al.Inducing protective immunity of a recombinant pseudorabies virus expressing the GP5 of porcine reproductive and respiratory syndrome virus in piglet[J].Vet Immunol Immunopathol,2005,106:309-319.
  • 7Ostrowski M,Galeota J A,Jar A M,et al.Identification of Neutralizing and Nonneutralizing Epitopes in the Porcine Reproductive and Respiratory Syndrome Virus GP5 Ectodomain[J].J Virol,76:4241-4250.
  • 8Ansari I H,Kwon B J,Osorio F A,et al.Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity,antigenicity,and ability to induce neutralizing antibodies[J].J Virol,2006,3994-4004.
  • 9Johnson C R,Yu Wanqin,Murtaugh M P.Cross-reactive antibody responses to nspl and Nsp2 of porcine reproductive and respiratory syndrome virus[J].J Gen Virol,2007,88:1184-1195.
  • 10De Lima M,Pattnaik A K,Flores E F,et al.Serologic marker candidates identified among B-cell linear epitopes of Nsp2 and structural proteins of a North American strain of porcine reproductive and respiratory syndrome virus[J].Virology,2006,353:410-421.

共引文献426

同被引文献6

引证文献1

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部