摘要
目的 了解儿童急性呼吸道感染病原分布情况,并评估多重聚合酶链反应(PCR)技术的临床应用价值。方法 收集2018年1月–2019年1月北京地区多个医院268例呼吸道感染患儿的咽拭子及支气管肺泡灌洗液,采用多重PCR技术测定13种病原的核酸,同时应用荧光定量PCR法检测肺炎支原体核酸,分析儿童急性呼吸道感染病原分布情况,并比较两种检测方法的一致性。结果 268例呼吸道标本,231例(86.19%)检出至少一种以上的病原,51例(19.03%)检出两种或两种以上病原。肺炎支原体(MP)和流感病毒占所检病原的前两位,检出的病原体按例数分别为MP109例、H1N143例、InfA36例、RSV 29例、H3N2 14例、HRV 14例、HMPV 13例、InfB 10例、HAd V 9例、HCoV 6例、HPIV 5例、HBoV1例、CP1例。多重PCR方法检测出MP阳性109例(40.67%),实时定量PCR法检测出MP阳性123例(45.90%),两种检测方法阳性率比较差异无统计学意义(P=0.262),一致性分析显示两种检测方法具有高度的一致性(Kappa=0.879)。结论 MP及流感病毒是北京地区儿童呼吸道感染的重要病原,而且混合感染的情况并不少见。对于MP,多重PCR技术和实时荧光定量PCR法敏感性基本一致,两者具有高度的一致性,多重PCR技术可同时检测病毒、MP、CP等多种病原体,特异性强,可提高样本使用率,具有良好的应用价值。
Objective To investigate the pathogen distribution of acute respiratory infection in children and evaluate the clinical application value of multiplex polymerase chain reaction(PCR).Methods The throat swabs and bronchoalveolar lavage fluid of 268 children with respiratory tract infection in multiple hospitals in Beijing from January 2018 to January 2019 were collected. The nucleic acids of 13 pathogens were determined by multiplex PCR,and the nucleic acids of Mycoplasma pneumoniae(MP) were detected by fluorescence quantitative PCR. The distribution of pathogens in children with acute respiratory tract infection was analyzed, and the consistency of the two detection methods was compared.Results Among 268 respiratory tract specimens, 231(86.19%) were detected to carry at least one pathogen, and 51(19.03%) were detected to carry with two or more pathogens. MP and influenza virus were detected as the top two pathogens. The number of patients carrying MP, H1N1, InfA, RSV, H3N2, HRV, HMPV, InfB, HAdV, HCoV, HPIV, HBoV and CP pathogen was 109, 43, 36,29, 14, 14, 13, 10, 9, 6, 5, 1 and 1 case(s), respectively. MP was detected in 109 cases(40.67%) by multiplex PCR and in 123 cases(45.90%) by real-time quantitative PCR. There was no significant difference in the positive rate between the two methods(P = 0.262).Consistency analysis showed that both methods had high consistency(Kappa = 0.879).Conclusion MP and influenza virus are important pathogens of respiratory tract infection in children in Beijing, and mixed infection is not uncommon. The sensitivity of multiplex PCR analysis is similar with MP real-time fluorescence quantitative PCR results, with high consistence between both methods. Multiplex PCR technology can simultaneously detect viruses, MP, CP and other pathogens with high specificity, which can improve the sample utilization rate and has good application value.
作者
袁青
史大伟
窦海伟
辛德莉
YUAN Qing;SHI Da-wei;DOU Hai-wei;XIN De-li(Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Disease,Beijing Tropical Medicine Research Institute,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)
出处
《中国医药生物技术》
2022年第6期512-517,共6页
Chinese Medicinal Biotechnology
关键词
呼吸道病原
多重PCR检测
肺炎支原体
儿童
respiratory pathogen
multiplex PCR detection
mycoplasma pneumonia
child
