摘要
目的探讨假基因MTND4P12在肝细胞癌中的表达及临床意义。方法采用癌症基因组图谱(TCGA)数据库中提供的临床和RNA-seq数据,以预测肝细胞癌可能标志物MTND4P12及其调控靶基因。随后利用免疫组织化学染色、蛋白免疫印迹等技术对本院肝癌患者样本中MTND4P12及其靶基因表达情况进行检测和验证。最后采用肝癌细胞基因干扰和过表技术,进一步验证MTND4P12在肝癌中的作用。结果MTND4P12在肝细胞癌组织中的表达阳性率为78.00%,明显高于癌旁组织(32.00%),组间比较差异具有统计学意义(P<0.001);在MTND4P12阳性组中,31例(79.49%)合并肝转移(P<0.001),且患者的TNM分期多为Ⅳ期患者,占84.62%,与MTND4P12阴性组比较差异具有统计学意义(P<0.001)。Kaplan-Meier生存分析表明,MTND4P12高表达与预后不良有关,且MTND4P12与癌基因PSMD11的表达呈正相关。同时,细胞实验证明,MTND4P12可使肝癌细胞增殖能力上升,迁移能力增强,伤口愈合能力增强。结论假基因MTND4P12在肝细胞癌组织中异常表达,影响患者预后,其机制可能与调控癌基因PSMD11的表达有关。
Objective To investigate the expression and clinical significance of pseudogene MTND4P12 in hepatocellular carcinoma(HCC).Methods The clinical and RNA-seq data from The Cancer Genome Atlas(TCGA)database were used to predict the possible marker of hepatocellular carcinoma MTND4P12 and its regulatory target genes.Then,the expression of MTND4P12 and its target genes in the samples of patients with HCC in our hospital were tested and verified by immunohistochemical staining and Western blot and other techniques.Finally,the role of MTND4P12 was verified by gene interference and overexpression techniques of liver cancer cells.Results The positive rate of MTND4P12 expression in HCC tissues(78.00%)was significantly higher than their adjacent tissues(32.00%);The difference between the two groups was statistically significant(P<0.001).In the MTND4P12 positive group,there were 31 cases(79.49%)with liver metastasis(P<0.001).The TNM stage of patients was mostlyⅣ(84.62%),which was statistically significant compared with the MTND4P12 negative group(P<0.001).Kaplan Meier survival analysis showed that the high expression of MTND4P12 was associated with poor prognosis,and the expression of MTND4P12 was positively correlated with the expression of oncogene PSMD11.And cell experiments proved that MTND4P12 enhanced the abilities of proliferation,migration and wound healing of HCC cells.Conclusion The abnormal expression of pseudogene MTND4P12 in HCC affects the prognosis of patients,and its mechanism may be related to the regulation of the expression of oncogene PSMD11.
作者
徐婷
华高艳
Xu Ting;Hua Gaoyan(Anhui Health College,Chizhou 247099,Anhui,China;Department of Oncology,The People’s Hospital of Chizhou,Chizhou 247099,Anhui,China)
出处
《右江民族医学院学报》
2022年第5期697-702,共6页
Journal of Youjiang Medical University for Nationalities
基金
安徽省高校优秀青年人才支持计划重点项目(gxyqZD2019124)
安徽省级示范基层教学组织(教研室)项目(2020SJSFJXZZ196)。
