摘要
目的探讨应用横向主动脉缩窄(transverse aortic constriction,TAC)术建立兔心力衰竭模型的方法及可行性。方法25只普通级健康雄性日本大耳兔,随机分为手术组(n=15)和假手术组(n=10)。手术组动物行TAC术,建立兔心力衰竭模型;假手术组行开胸手术但不进行缩窄操作。术后4周和8周超声检查心脏功能,术后8周检测降主动脉流速;结合分子生物学指标及病理学观察,评估TAC术建立兔心力衰竭模型的可行性和稳定性。结果TAC术后8周,手术组兔存活13只,假手术组兔存活10只,手术组主动脉弓缩窄处血流速度均大于2.20 m/s。与假手术组比较,手术组心率、升主动脉内径、左房舒张末期内径、左室舒张末期内径和左室收缩末期内径均明显增加(P<0.05),左室射血分数明显降低(P<0.05);与假手术组相比,手术组左心室组织中NT-proBNP和β-MHC含量明显升高(P<0.05)。病理切片显示,TAC术后8周,手术组心肌细胞肥大且排列紊乱,心肌纤维束增宽。TAC术后4周,与假手术组相比,手术组左室舒张末期室间隔厚度和左室后壁舒张末期厚度均显著增加(P<0.05)。结论应用TAC术建立实验兔心力衰竭模型是有效且可行的。心脏超声是评估主动脉弓缩窄程度及心力衰竭建模成功与否的有效手段。手术组实验兔在TAC术后8周时心肌出现失代偿反应,形成不可逆的心力衰竭。
Objective To investigate the method and feasibility of establishing a rabbit heart-failure model using transverse aortic constriction(TAC). Methods Twenty-five healthy male Japanese white rabbits were randomly divided into an operation group(n=15) and a sham-operation group(n=10). The animals in the operation group underwent TAC to establish the rabbit model of heart failure. The shamoperation group underwent thoracotomy but no narrowing operation. Cardiac function was evaluated using ultrasound at 4 and 8 weeks after operation. Eight weeks after operation, the descending aortic flow velocity was detected using cardiac ultrasound, combined with molecular biological indexes and pathological observations to verify the feasibility and stability of TAC in establishing the rabbit heartfailure model. Results Eight weeks after operation, 13 rabbits in the operation group and 10 in the sham-operation group survived. The blood flow velocities at the constriction in the operation group were >2.20 m/s. Compared with the sham-operation group, the following outcomes in the operation group increased significantly(P<0.05): heart rate, ascending aortic diameter, left atrial end-diastolic diameter, left ventricular end-diastolic diameter, and left ventricular end-systolic diameter. Left ventricular ejection fraction decreased significantly(P<0.05). Compared with the sham-operation group, the contents of left ventricular N-terminal pro B type natriuretic peptide(NT-proBNP) andβ-myosin heavy chain(β-MHC)significantly increased in the operation group(P<0.05). Pathological sections showed that 8 weeks after TAC, cardiomyocytes in the operation group were hypertrophic and disordered, and myocardial fiber bundles widened. Interventricular septal end-diastolic thickness and left ventricular posterior wall enddiastolic thickness significantly increased in the operation group(P<0.05), compared to those in the shamoperation group at 4 weeks. Conclusion It is effective and feasible to establish an experimental rabbit heart-failure model using TAC. Cardiac ultrasound is an effective method to evaluate and determine the degree of aortic arch constriction and the success of heart-failure modeling. Rabbits in the operation group showed myocardial decompensation at 8 weeks after TAC, resulting in irreversible heart failure.
作者
罗庆祎
章体玲
丁云川
陈剑
赵丽
王庆慧
LUO Qingyi;ZHANG Tiling;DING Yunchuan;CHEN Jian;ZHAO Li;WANG Qinghui(Department of Ultrasound Medicine,Yan'an Hospital Affiliated to Kunming Medical University,Kunming 650200,China;Key Laboratory of Cardiovascular Diseases of Yunnan Province,Kunming 650200,China;Medical Center of Heart Disease of Yunnan Province,Kunming 650200,China;Department of Cardiology Medicine,Yan'an Hospital Affiliated to Kunming Medical University,Kunming 650200,China)
出处
《实验动物与比较医学》
CAS
2022年第5期401-408,共8页
Laboratory Animal and Comparative Medicine
基金
云南省科技厅科技计划项目“围术期多模态超声心动图在冠状动脉旁路移植术中的应用研究”(2019FE001-266)。
关键词
超声
横向主动脉缩窄术
心力衰竭
兔
Ultrasound
Transverse aortic constriction
Heart failure
Rabbits
