摘要
目的:探究佐剂性关节炎(AA)大鼠外周血单个核细胞(PBMCs)中人微小RNAs(hsa-miRNAs)的表达及新风胶囊(XFC)干预研究。方法:建立AA大鼠模型,造模后第12天开始给药,将大鼠随机分为正常组(NC组)、模型组(MC组)、XFC低剂量组(L-XFC组)、XFC中剂量组(M-XFC组)、XFC高剂量组(H-XFC组)。给药30 d后,观察各组大鼠体质量、足跖肿胀度、关节炎指数(AI)的变化;RT-qPCR法检测PBMCs中miRNAs的表达;ELISA法检测血清中C反应蛋白(CRP)、抗环瓜氨酸肽抗体(抗CCP抗体)、类风湿因子(RF)、白细胞介素(IL)-11、IL-17、IL-4、IL-10的表达。结果:①致炎前,各组大鼠体质量、足跖肿胀度及AI比较,差异无统计学意义(P>0.05);致炎12 d,各组大鼠足跖肿胀度和AI均显著升高(P<0.01);XFC给药后,各组大鼠体质量、足跖肿胀度、AI得到显著改善。②与NC组相比,MC组hsa-miR-146a-3p、hsa-miR-31-5p、hsa-miR-29c-3p、hsa-miR-597-3p mRNA表达显著降低(P<0.01),CRP、RF、抗CCP抗体、IL-17、hsa-miR-3184-5p mRNA表达显著升高(P<0.01);XFC治疗后,上述指标得到显著改善。③Spearman相关性分析结果显示,hsa-miR-146a-3p与足跖肿胀度、CRP、RF、IL-11呈显著负相关,与IL-4呈显著正相关(P<0.05);hsa-miR-31-5p与AI、RF、IL-17呈负相关,与IL-10呈正相关(P<0.05);hsa-miR-29c-3p与RF、IL-11呈负相关(P<0.05);hsa-miR-3184-5p与IL-4呈负相关(P<0.05)。结论:AA大鼠PBMCs中存在差异表达的hsa-miRNAs,与免疫炎症指标具有显著相关性,XFC在改善免疫炎症指标的同时,也能改善miRNAs,其机制有待进一步研究。
Objective:To investigate the expression of hsa-miRNAs in peripheral blood mononuclear cells(PBMCs)of adjuvant arthritis(AA)rats and the intervention of Xinfeng Capsule(新风胶囊,XFC).Methods:The AA rat model was established,and the drug was administered on the 12 th day after the model was established.The rats were randomly divided into a normal group(NC group),a model group(MC group),a low-dose XFC group(L-XFC group),a medium-dose XFC group(M-XFC group),and a high-dose XFC group(H-XFC group).After 30 days of administration,the changes of body mass,footpad swelling,and arthritis index(AI) of the rats in each group were observed.The expression of miRNAs in PBMCs was detected by RT-qPCR.The expressions of CRP,anti CCP antibody,RF,interleukin IL-11,IL-17,IL-4 and IL-10 in serum were detected by ELISA.Results: ① There was no significant difference(P > 0.05)in body mass,footpad swelling and AI of rats in each group before inflammation;twelve days after inflammation,the degree of footpad swelling and AI of rats in each group increased significantly(P < 0.01);After administration of XFC,body mass,footpad swelling and AI were significantly improved. ② Compared with the NC group,the expressions of hsa-miR-146a-3p,hsa-miR-31-5p, hsa-miR-29c-3p,and hsa-miR-597-3p mRNA in the MC group decreased significantly(P < 0.01),while the expressions of CRP,RF,anti CCP antibody,IL-17,hsa-miR-3184-5p mRNA in the MC group increased significantly (P < 0.01);After treatment with XFC,the above indicators were significantly improved. ③ Spearman correlation analysis showed that hsa-miR-146a-3p was significantly negatively correlated with footpad swelling,CRP,RF and IL-11,and significantly positively correlated with IL-4(P < 0.05);hsa-miR-31-5p was negatively correlated with AI,RF,IL-17,and positively correlated with IL-10(P < 0.05);hsa-miR-29c-3p was negatively correlated with RF and IL-11(P < 0.05);and there was a negative correlation between hsa-miR-3184-5p and IL-4(P < 0.05). Conclusion:There are hsa-miRNAs differentially expressed in PBMCs of AA rats,which are significantly correlated with immune inflammatory indicators.XFC can improve both immune inflammatory indicators and miRNAs,and its mechanism needs further study.
作者
文建庭
刘健
姜辉
孙艳秋
王馨
王杰
周琴
丁香
陈晓露
张先恒
WEN Jian-ting;LIU Jian;JIANG Hui;SUN Yan-qiu;WANG Xin;WANG Jie;ZHOU Qin;DING Xiang;CHEN Xiao-lu;ZHANG Xian-heng
出处
《风湿病与关节炎》
2022年第10期1-6,共6页
Rheumatism and Arthritis
基金
国家自然科学基金面上项目(81973655,82074373)
科技部国家重点研发计划中医药现代化研究重点专项(2018YFC1705204)
安徽省高校协同创新项目(GXXT-2020-025)
安徽省重点研究和开发计划对外科技合作项目(201904b11020011)
安徽省名中医刘健工作室建设项目(中医药发展秘〔2018〕11号)
安徽省重点研究与开发计划项目(201904a07020004)
安徽现代中医内科应用基础与开发研究省级实验室专项(2016080503B041)
安徽省第12批“115”创新团队项目(皖人才办〔2019〕1号)
新安医学教育部重点实验室开放基金项目(2020xayx10)。
