摘要
目的 研究川西高原上野生川赤芍Paeonia veitchii入药部位(根)和非入药部位(地上部分)中没食子酸、氧化芍药苷、儿茶素、芍药内酯苷、芍药苷、苯甲酸和丹皮酚7种化学成分的含量,并综合评价其品质。方法 以上述7种成分为对照品,采用高效液相色谱(HPLC)法,Agilent HC-C18色谱柱(250 mm×4.6 mm,5μm),柱温30℃,体积流量1 mL/min,进样量10μL,检测波长230 nm,乙腈-0.1%磷酸水梯度洗脱;采用2012版《中药色谱指纹图谱相似度评价系统》软件构建指纹图谱;采用聚类热图分析、相似度分析、主成分分析(principal component analysis,PCA)、偏最小二乘回归分析(partial leastsquares discrimination analysis,PLS-DA)等方法对各产地川赤芍进行评价。结果 野生川赤芍地上部分和根中均含有7种化学成分,仅有3批次地上部分样品中芍药苷含量不符合《中国药典》2020年版的要求。建立了野生川赤芍根和地上部分的HPLC指纹图谱,二者均标定了19个相同的共有峰,并指认出其中的7个(没食子酸、氧化芍药苷、儿茶素、芍药内酯苷、芍药苷、苯甲酸、丹皮酚)。不同产地川赤芍的化学成分种类和含量存在一定差异且存在明显的区域一致性特征,但芍药苷的差异相对较小。康定市老榆林、雅江县相格宗、阿坝县查理加油站和若尔盖县巴西乡的川赤芍药材质量相对较好。结论 建立的HPLC方法可用于分析川赤芍中的主要化合物,川西高原野生川赤芍药材质量符合《中国药典》要求,川赤芍根和地上部分中化学成分的种类和含量相近,地上部分具有开发利用的潜力。
Objective To determine the contents of gallic acid, oxypaeoniflorin, catechin, albiflorin, paeoniflorin, benzoic acid and paeonol of the roots and aerial parts of wild P. veitchii from the Western Sichuan plateau, and then comprehensively evaluate of the quality of P. veitchii.Methods High performance liquid chromatography(HPLC) analysis was used to analyze the samples with the following conditions: column type, agilent HC-C18 column(250 mm× 4.6 mm, 5 μm);temperature, 30 °C;flow rate, 1 mL/min;injection volume, 10 μL;wavelength, 230 nm;and gradually diluted with acetonitrile-0.1% phosphoric acid water. The fingerprints were established by the Similarity Evaluation System of Traditional Chinese Medicine(version 2012). The comprehensive evaluation of the quality of P. veitchii was performed by the hierarchical clustering heat map analysis, similarity analysis, partial least squares discrimination analysis(PLS-DA) and principal component analysis(PCA).Results The results showed that both roots and aerial parts of P. veitchii contained gallic acid, oxypaeoniflorin, catechin, albiflorin, paeoniflorin,benzoic acid and paeonol. And the content of paeoniflorin in only three samples did not meet the requirement of the 2020 edition of China Pharmacopoeia. The HPLC fingerprints of roots and aerial parts of P. veitchii were established respectively, and both of the two fingerprints had 19 common peaks, of which seven were identified as gallic acid, oxypaeoniflorin, catechin, albiflorin, paeoniflorin, benzoic acid and paeonol.The kinds and contents of chemical components in both roots and aerial parts of P. veitchii from different areas were variable, but the variation of paeoniflorin was relatively small. There was a feature of obvious regional consistency in the kinds and contents of chemical components of P.veitchii. Moreover, the rhizome(medicinal part) quality of P. veitchii from Laoyulin of Kangding City, Xianggezong of Yajiang County, Chali gas station of Aba County and Baxixiang of Ruoergai County was relatively better. Conclusion HPLC method established in this study could simultaneously separate and analyze the main compounds in P. veitchii. The rhizome quality of P. veitchii from the Western Sichuan plateau met the requirement of Chinese Pharmacopoeia. Because the kinds and contents of chemical components in roots and aerial parts of P. veitchii were similar, the aerial parts had the potentiality of development and utilization.
作者
曲别军长
俸明康
张绍山
兰建龙
海来约布
黄艳菲
杨正明
李文兵
陈晨
李莹
刘璇
刘圆
QUBIE Jun-zhang;FENG Ming-kang;ZHANG Shao-shan;LAN Jian-long;HAILAI Yue-bu;HUANG Yan-fei;YANG Zheng-ming;LI Wen-bing;CHEN Chen;LI Ying;LIU Xuan;LIU Yuan(College of Pharmacy,Southwest Minzu University,Chengdu 610041,China;Institute of Qinghai-Tibetan Plateau,Southwest Minzu University,Chengdu 610041,China;Sichuan Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory,Chengdu 610225,China;Key Laboratory of Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization of National Ethnic Affairs Commission of the People's Republic of China,Chengdu 610225,China;Ethnic Medicine Institute,Southwest Minzu University,Chengdu 610225,China)
出处
《中草药》
CAS
CSCD
北大核心
2022年第18期5842-5850,共9页
Chinese Traditional and Herbal Drugs
基金
国家重点研发计划(2018YFC1708005)
四川省科技计划项目(重点研发项目-2021YFS0043)
西南民族大学2022年研究生创新型项目资助硕士重点项目(ZD2022491)。
关键词
野生川赤芍
高效液相色谱
没食子酸
氧化芍药苷
儿茶素
芍药内酯苷
芍药苷
苯甲酸
丹皮酚
品质评价
wild Paeonia veitchii Lynch
high performance liquid chromatography
gallic acid
oxypaeoniflorin
catechin
albiflorin
paeoniflorin
benzoic acid
paeonol
quality evaluation
