摘要
[目的]以701份杜仲种质资源为研究对象,探究杜仲种质资源叶片表型性状遗传多样性,为杜仲优良资源选育和开发利用提供理论支撑和材料。[方法]对杜仲叶片14个表型性状进行测定,结合相关性分析、主成分分析和聚类分析等统计方法,探究杜仲不同种质间叶片表型的遗传多样性。[结果]不同来源地杜仲叶片的14个表型性状间存在较大差异,叶面积变异系数最大(20.68%),叶绿素参考值变异系数最小(4.57%);各表型性状的遗传多样性指数为1.90~2.09,叶片长和叶柄长的遗传多样性指数最大,均为2.09;叶片表型性状间呈极显著正相关的有26对,呈极显著负相关的有19对,叶片长、叶片宽、叶面积、叶周长、叶柄长5个性状间均呈极显著正相关。主成分分析提取的6个主成分,累积贡献率达89.211%。基于14个表型性状,将701份杜仲种质划分为4类,各类群间叶片表型性状存在较大差异。[结论]杜仲种质资源叶片表型性状表现出丰富的多样性,筛选出叶面积、鲜比叶质量、叶片绿色成分、叶绿素参考值、长宽比、节间距6个性状可作为评定杜仲资源的主要表型指标,为叶用杜仲良种选育及综合利用提供了优异的种质基础。
[Objective]The genetic diversity of leaf phenotypic traits of 701 Eucommia ulmoides germplasm resources was investigated to provide theoretical support and materials for the breeding,development and utilization of Eucommia ulmoides.[Methods]Fourteen phenotypic traits of Eucommia ulmoides leaves were determined.Correlation analysis,principal component analysis and cluster analysis were used to investigate the genetic diversity of leaf phenotypic traits among different germplasm.[Results]There were significant differences among 14 phenotypic traits of Eucommia ulmoides leaves.The coefficient of variation of leaf area was the largest(20.68%),and the coefficient of variation of chlorophyll reference value was the smallest(4.57%).The genetic diversity index of each phenotypic trait ranged from 1.90 to2.09,and the genetic diversity index of leaf length and petiole length was the highest with 2.09.There were 26 pairs of leaf phenotypic traits that are significantly positively correlated,and 19 pairs that are significantly negatively correlated(P<0.01).There were extremely significant positive correlations among the five traits of leaf length,leaf width,leaf area,leaf circumference and petiole length(P<0.01).The cumulative contribution rate of 6 principal components extracted by principal component analysis was 89.211%.Based on 14 phenotypic traits,701 Eucommia ulmoides germplasm were divided into 4 categories.There were significant differences in leaf phenotypic traits among various groups.[Conclusion]The leaf phenotypic traits of Eucommia ulmoides germplasm resources show rich diversity,and six traits are selected as important indicators for evaluating the resources of Eucommia ulmoides germplasm resources,including leaf area,Specific leaf fresh weight,green component of leaf,chlorophyll reference value,length to width ratio of leaf,and internode length.It provides an excellent germplasm basis for the selection and comprehensive utilization of Eucommia ulmoides varieties.
作者
孟益德
杜红岩
王璐
吕庚鑫
庆军
何凤
黄海燕
杜庆鑫
MENG Yi-de;DU Hong-yan;WANG Lu;LV Geng-xin;QING Jun;HE Feng;HUANG Hai-yan;DU Qing-xin(Research Institute of Non-Timber Forestry,Chinese Academy of Forestry,Zhengzhou 450003,He’nan,China;The Eucommia Engineering Research Center,State Administration of Forestry and Grassland,Zhengzhou 450003,He’nan,China;He’nan Runyou Forestry Technical Service Co.Ltd,Zhengzhou 450003,He’nan,China)
出处
《林业科学研究》
CSCD
北大核心
2022年第5期103-112,共10页
Forest Research
基金
“十三五”国家重点研发计划项目“橡胶用和药用杜仲定向培育与高效利用关键技术研究”(2017YFD0600702)
国家自然科学基金面上项目(31370682)。
关键词
杜仲叶片
种质资源
表型性状
多样性
Eucommia ulmoides leaves
germplasm resources
phenotypic traits
diversity
