摘要
Objective:To investigate the effect of small nucleolar host gene 12(SNHG12)on the migration and invasion of human lung cancer cells by regulating human angiostatin binding protein through microribonucleic acid(microRNA)-497.Methods:A549,H1299,and PC9 cells were cultured in Roswell Park Memorial Institute(RPMI)-1640 medium containing 10%fetal bovine serum,and human bronchial epithelial(HBE)cells were cultured in Dulbecco’s modified eagle medium(DMEM)containing 10%fetal bovine serum.The incubator conditions were as follows:saturated humidity,37℃,and 5%carbon dioxide(CO2).Results:The gene expressions of small nucleolar host gene 12(SNHG12)in HBE,A549,H1299,and PC9 were 1.00±0.02,5.61±0.42,3.78±0.29,and 3.51±0.23,respectively.The gene expressions of microRNA-497HBE,A549,H1299,and PC9 were 1.00±0.13,0.21±0.04,0.35±0.05,and 0.37±0.06,respectively,with P<0.05.The microRNA-497 gene expression and cell apoptosis rate in the microRNA-497 group and the microRNA-497+pcDNA3.1 group were significantly higher than those in the miR-NC group,whereas the A value and cell invasion number were significantly lower than those in the miR-negative control(NC)group,with P<0.05.Compared with the microRNA-497+pcDNA3.1 group,the microRNA-497 gene expression and cell apoptosis rate in the microRNA-497+SNHG12 group were significantly lower,whereas the A value and cell invasion number were significantly higher,with P<0.05.Conclusion:SNHG12 can inhibit the migration and invasion of human lung cancer cells by regulating human angiostatin binding protein through microRNA-497.
基金
supported by the Baoding Science and Technology Planning Project(Grant Number:2141ZF127).
